pyruvate_minimal_media

CultureMech:007020 bacterial DEFINED LIQUID
Original name: pyruvate minimal media

Overview

External ontology
MEDIADB:131 (source link unavailable) — pyruvate minimal media
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007020["pyruvate_minimal_media"]
    ing0_Pyruvate["Pyruvate"]
    CultureMech_007020 --> ing0_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing0_Pyruvate -.-> CHEBI_15361
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007020 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007020 --> ing2_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing3_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007020 --> ing3_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing3_Sodium_molybdate -.-> CHEBI_75215
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_007020 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007020 --> ing5_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_HCl -.-> CHEBI_49105
    ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007020 --> ing6_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing7_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007020 --> ing7_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing7_Magnesium_sulfate -.-> CHEBI_32599
    ing8_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007020 --> ing8_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing8_Cobalt_chloride -.-> CHEBI_35696
    ing9_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007020 --> ing9_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing9_Ammonium_sulfate -.-> CHEBI_62946
    ing10_Manganese_chloride["Manganese chloride"]
    CultureMech_007020 --> ing10_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing10_Manganese_chloride -.-> CHEBI_63041
    ing11_Cupric_chloride["Cupric chloride"]
    CultureMech_007020 --> ing11_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing11_Cupric_chloride -.-> CHEBI_49553
    ing12_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007020 --> ing12_Iron_III__chloride
    ing13_Zinc_Chloride["Zinc Chloride"]
    CultureMech_007020 --> ing13_Zinc_Chloride
    CHEBI_49976["CHEBI:49976"]
    ing13_Zinc_Chloride -.-> CHEBI_49976

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Pyruvate
C3H3O3 87.054 g/mol
56.779 MILLIMOLAR CHEBI:15361
pyruvate
 Cross-references: KEGG:pyr
2 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.04966 MILLIMOLAR CHEBI:3312
calcium dichloride
3 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
48.0 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
4 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.00248 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
5 Sodium chloride
Cl.Na 58.443 g/mol
10.0 MILLIMOLAR CHEBI:26710
sodium chloride
6 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
3.32e-06 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
7 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
22.0 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
8 Magnesium sulfate
Mg.O4S 120.368 g/mol
1.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
9 Cobalt chloride
2Cl.Co 129.839 g/mol
0.00252 MILLIMOLAR CHEBI:35696
cobalt dichloride
10 Ammonium sulfate
2H4N.O4S 132.141 g/mol
30.0 MILLIMOLAR CHEBI:62946
ammonium sulfate
11 Manganese chloride
Cl2Mn 125.844 g/mol
0.00448 MILLIMOLAR CHEBI:63041
manganese(II) chloride
12 Cupric chloride
Cl2Cu 134.452 g/mol
0.00252 MILLIMOLAR CHEBI:49553
copper(II) chloride
13 Iron(III) chloride 0.06165 MILLIMOLAR
14 Zinc Chloride
2Cl.Zn 136.296 g/mol
0.01247 MILLIMOLAR CHEBI:49976
zinc dichloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.774713Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 131, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:45.629824Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007020
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.724194+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "pyruvate minimal media" to "pyruvate_minimal_media" for consistent matching
2026-06-05T07:41:43.506448+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:07:08.727506+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:131's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.