glucose_minimal_media_zhao_zhu

CultureMech:007021 bacterial DEFINED LIQUID
Original name: glucose minimal media (zhao/zhu)

Overview

External ontology
MEDIADB:132 (source link unavailable) — glucose minimal media (zhao/zhu)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007021["glucose_minimal_media_zhao_zhu"]
    ing0_beta_D_Glucose["beta-D-Glucose"]
    CultureMech_007021 --> ing0_beta_D_Glucose
    CHEBI_15903["CHEBI:15903"]
    ing0_beta_D_Glucose -.-> CHEBI_15903
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007021 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007021 --> ing2_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing3_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007021 --> ing3_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing3_Sodium_sulfate -.-> CHEBI_32149
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007021 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007021 --> ing5_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing5_Manganese_sulfate -.-> CHEBI_86364
    ing6_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007021 --> ing6_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing6_Ammonium_chloride -.-> CHEBI_31206
    ing7_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007021 --> ing7_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing7_Cobalt_chloride -.-> CHEBI_35696
    ing8_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007021 --> ing8_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing8_Zinc_sulfate -.-> CHEBI_35176
    ing9_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
    CultureMech_007021 --> ing9_Sodium_dihydrogen_phosphate
    CHEBI_37585["CHEBI:37585"]
    ing9_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
    ing10_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007021 --> ing10_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing10_Ammonium_sulfate -.-> CHEBI_62946
    ing11_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007021 --> ing11_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing11_Cupric_sulfate -.-> CHEBI_23414
    ing12_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007021 --> ing12_Iron_III__chloride
    ing13_Sodium_EDTA["Sodium EDTA"]
    CultureMech_007021 --> ing13_Sodium_EDTA

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 beta-D-Glucose
C6H12O6 180.156 g/mol
55.506 MILLIMOLAR CHEBI:15903
beta-D-glucose
2 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.01163 MILLIMOLAR CHEBI:3312
calcium dichloride
3 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
41.9058 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
4 Sodium sulfate
2Na.O4S 142.043 g/mol
14.0449 MILLIMOLAR CHEBI:32149
sodium sulfate
5 Magnesium sulfate
Mg.O4S 120.368 g/mol
3.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
6 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.001775 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
7 Ammonium chloride
Cl.H4N 53.492 g/mol
9.3474 MILLIMOLAR CHEBI:31206
ammonium chloride
8 Cobalt chloride
2Cl.Co 129.839 g/mol
0.00227 MILLIMOLAR CHEBI:35696
cobalt dichloride
9 Zinc sulfate
O4S.Zn 161.453 g/mol
0.001878 MILLIMOLAR CHEBI:35176
zinc sulfate
10 Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
30.005 MILLIMOLAR CHEBI:37585
sodium dihydrogenphosphate
11 Ammonium sulfate
2H4N.O4S 132.141 g/mol
18.919 MILLIMOLAR CHEBI:62946
ammonium sulfate
12 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0019223 MILLIMOLAR CHEBI:23414
copper(II) sulfate
13 Iron(III) chloride 0.18535 MILLIMOLAR
14 Sodium EDTA 0.18828 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.783575Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 132, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:45.635982Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007021
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.730973+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'glucose minimal media (zhao/zhu" to "glucose_minimal_media_zhao_zhu" for consistent matching
2026-06-05T07:41:06.469642+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-09T01:42:20.640295+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-20T19:01:50.565139+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-08-31T04:06:21.367779+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:132's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.
2026-08-31T06:17:32.852402+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.