Overview
- External ontology
- MEDIADB:135 (source link unavailable) — M63 with thiamine-hcl with fructose
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007024["m63_with_thiamine_hcl_with_fructose"]
ing0_D_Fructose["D-Fructose"]
CultureMech_007024 --> ing0_D_Fructose
CHEBI_15824["CHEBI:15824"]
ing0_D_Fructose -.-> CHEBI_15824
ing1_Sodium_hydroxide["Sodium hydroxide"]
CultureMech_007024 --> ing1_Sodium_hydroxide
CHEBI_32145["CHEBI:32145"]
ing1_Sodium_hydroxide -.-> CHEBI_32145
ing2_Thiamine_HCl["Thiamine HCl"]
CultureMech_007024 --> ing2_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing2_Thiamine_HCl -.-> CHEBI_49105
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007024 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007024 --> ing4_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing4_Ferrous_sulfate -.-> CHEBI_75832
ing5_Sodium_dihydrogen_phosphate["Sodium dihydrogen phosphate"]
CultureMech_007024 --> ing5_Sodium_dihydrogen_phosphate
CHEBI_37585["CHEBI:37585"]
ing5_Sodium_dihydrogen_phosphate -.-> CHEBI_37585
ing6_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007024 --> ing6_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing6_Ammonium_sulfate -.-> CHEBI_62946
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Fructose
C6H12O6 180.156 g/mol
|
22.0 MILLIMOLAR |
CHEBI:15824
D-fructose
|
Cross-references: KEGG:fru |
| 2 |
Sodium hydroxide
HO.Na 39.997 g/mol
|
75.0 MILLIMOLAR |
CHEBI:32145
sodium hydroxide
|
|
| 3 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.0033 MILLIMOLAR |
CHEBI:49105
thiamine hydrochloride
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.16 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.0039 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 6 |
Sodium dihydrogen phosphate
H2O4P.Na 119.976 g/mol
|
11.0 MILLIMOLAR |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 7 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
15.0 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.808576Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 135, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.649810Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007024 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.746445+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M63 with thiamine-hcl with fructose" to "m63_with_thiamine_hcl_with_fructose" for consistent matching |
| 2026-06-05T07:41:11.888778+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-09T01:42:21.064287+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |