Overview
- External ontology
- MEDIADB:137 (source link unavailable) — basal solution P
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- High-metal flag
- True
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007026["basal_solution_p"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007026 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007026 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007026 --> ing2_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing3_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007026 --> ing3_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing3_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing4_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007026 --> ing4_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing4_Magnesium_sulfate -.-> CHEBI_32599
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
22.0 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.09 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
94.0 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
100.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.001 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.824799Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 137, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.658235Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007026 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.755641+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "basal solution P" to "basal_solution_p" for consistent matching |
| 2026-06-05T07:40:50.575570+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |