mmgly_jasiecki_et_al

CultureMech:007028 bacterial DEFINED LIQUID
Original name: Mmgly; jasiecki et al

Overview

External ontology
MEDIADB:139 (source link unavailable) — Mmgly; jasiecki et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007028["mmgly_jasiecki_et_al"]
    ing0_Glycerol["Glycerol"]
    CultureMech_007028 --> ing0_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing0_Glycerol -.-> CHEBI_17754
    ing1_Thiamine["Thiamine"]
    CultureMech_007028 --> ing1_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing1_Thiamine -.-> CHEBI_18385
    ing2_Maleic_acid["Maleic acid"]
    CultureMech_007028 --> ing2_Maleic_acid
    CHEBI_18300["CHEBI:18300"]
    ing2_Maleic_acid -.-> CHEBI_18300
    ing3_Tromethamine["Tromethamine"]
    CultureMech_007028 --> ing3_Tromethamine
    CHEBI_9754["CHEBI:9754"]
    ing3_Tromethamine -.-> CHEBI_9754
    ing4_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007028 --> ing4_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing4_Magnesium_chloride -.-> CHEBI_86345
    ing5_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007028 --> ing5_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing5_Sodium_sulfate -.-> CHEBI_32149
    ing6_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007028 --> ing6_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing6_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing7_Sodium_chloride["Sodium chloride"]
    CultureMech_007028 --> ing7_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing7_Sodium_chloride -.-> CHEBI_26710
    ing8_Potassium_chloride["Potassium chloride"]
    CultureMech_007028 --> ing8_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing8_Potassium_chloride -.-> CHEBI_32588
    ing9_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007028 --> ing9_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing9_Ammonium_chloride -.-> CHEBI_31206

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Glycerol
C3H8O3 92.094 g/mol
21.7179 MILLIMOLAR CHEBI:17754
glycerol
 Cross-references: KEGG:glyc
2 Thiamine
C12H17N4OS 265.362 g/mol
0.037684 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
3 Maleic acid
C4H4O4 116.072 g/mol
49.9698 MILLIMOLAR CHEBI:18300
maleic acid
 Cross-references: KEGG:male
4 Tromethamine
C4H11NO3 121.136 g/mol
49.5295 MILLIMOLAR CHEBI:9754
tris
5 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
4.91859 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
6 Sodium sulfate
2Na.O4S 142.043 g/mol
0.929316 MILLIMOLAR CHEBI:32149
sodium sulfate
7 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
0.898939 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
8 Sodium chloride
Cl.Na 58.443 g/mol
42.7789 MILLIMOLAR CHEBI:26710
sodium chloride
9 Potassium chloride
Cl.K 74.551 g/mol
26.8272 MILLIMOLAR CHEBI:32588
potassium chloride
10 Ammonium chloride
Cl.H4N 53.492 g/mol
18.6947 MILLIMOLAR CHEBI:31206
ammonium chloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.841306Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 139, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:08.805242Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.669265Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007028
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.767732+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Mmgly; jasiecki et al" to "mmgly_jasiecki_et_al" for consistent matching
2026-06-05T07:41:35.432886+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.