Overview
- External ontology
- MEDIADB:144 (source link unavailable) — Mms medium; huber et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007034["mms_medium_huber_et_al"]
ing0_Starch["Starch"]
CultureMech_007034 --> ing0_Starch
CHEBI_28017["CHEBI:28017"]
ing0_Starch -.-> CHEBI_28017
ing1_Magnesium_chloride["Magnesium chloride"]
CultureMech_007034 --> ing1_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing1_Magnesium_chloride -.-> CHEBI_86345
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007034 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Potassium_iodide["Potassium iodide"]
CultureMech_007034 --> ing3_Potassium_iodide
CHEBI_8346["CHEBI:8346"]
ing3_Potassium_iodide -.-> CHEBI_8346
ing4_Resazurin["Resazurin"]
CultureMech_007034 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Boric_acid["Boric acid"]
CultureMech_007034 --> ing5_Boric_acid
CHEBI_33118["CHEBI:33118"]
ing5_Boric_acid -.-> CHEBI_33118
ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_007034 --> ing6_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
ing7_Sodium_molybdate["Sodium molybdate"]
CultureMech_007034 --> ing7_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing7_Sodium_molybdate -.-> CHEBI_75215
ing8_Sodium_chloride["Sodium chloride"]
CultureMech_007034 --> ing8_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing8_Sodium_chloride -.-> CHEBI_26710
ing9_Potassium_chloride["Potassium chloride"]
CultureMech_007034 --> ing9_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing9_Potassium_chloride -.-> CHEBI_32588
ing10_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007034 --> ing10_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing10_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing11_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007034 --> ing11_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing11_Magnesium_sulfate -.-> CHEBI_32599
ing12_Manganese_sulfate["Manganese sulfate"]
CultureMech_007034 --> ing12_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing12_Manganese_sulfate -.-> CHEBI_86364
ing13_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007034 --> ing13_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing13_Ferrous_sulfate -.-> CHEBI_75832
ing14_Cobalt_chloride["Cobalt chloride"]
CultureMech_007034 --> ing14_Cobalt_chloride
CHEBI_35696["CHEBI:35696"]
ing14_Cobalt_chloride -.-> CHEBI_35696
ing15_Zinc_sulfate["Zinc sulfate"]
CultureMech_007034 --> ing15_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing15_Zinc_sulfate -.-> CHEBI_35176
ing16_Cupric_sulfate["Cupric sulfate"]
CultureMech_007034 --> ing16_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing16_Cupric_sulfate -.-> CHEBI_23414
ing17_Aluminum_potassium_sulfate["Aluminum potassium sulfate"]
CultureMech_007034 --> ing17_Aluminum_potassium_sulfate
CHEBI_86463["CHEBI:86463"]
ing17_Aluminum_potassium_sulfate -.-> CHEBI_86463
ing18_Sodium_bromide["Sodium bromide"]
CultureMech_007034 --> ing18_Sodium_bromide
CHEBI_63004["CHEBI:63004"]
ing18_Sodium_bromide -.-> CHEBI_63004
ing19_Sodium_sulfide["Sodium sulfide"]
CultureMech_007034 --> ing19_Sodium_sulfide
CHEBI_76208["CHEBI:76208"]
ing19_Sodium_sulfide -.-> CHEBI_76208
ing20_Nickel_II__ammonium_sulfate["Nickel(II) ammonium sulfate"]
CultureMech_007034 --> ing20_Nickel_II__ammonium_sulfate
ing21_Strontium_chloride["Strontium chloride"]
CultureMech_007034 --> ing21_Strontium_chloride
CHEBI_36383["CHEBI:36383"]
ing21_Strontium_chloride -.-> CHEBI_36383
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Starch | 27.7531 MILLIMOLAR |
CHEBI:28017
starch
|
|
| 2 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
6.78766 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
3.43424 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Potassium iodide
I.K 166.002 g/mol
|
0.000150599 MILLIMOLAR |
CHEBI:8346
potassium iodide
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.004363 MILLIMOLAR |
CHEBI:8806
Resazurin
|
|
| 6 |
Boric acid
H3BO3 61.833 g/mol
|
0.123726 MILLIMOLAR |
CHEBI:33118
boric acid
|
|
| 7 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.117715 MILLIMOLAR |
CHEBI:44557
nitrilotriacetic acid
|
|
| 8 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.000619963 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 9 |
Sodium chloride
Cl.Na 58.443 g/mol
|
118.84 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 10 |
Potassium chloride
Cl.K 74.551 g/mol
|
2.14617 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 11 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
3.67414 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
7.28283 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 13 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.040098 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 14 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.00539471 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 15 |
Cobalt chloride
2Cl.Co 129.839 g/mol
|
0.0115527 MILLIMOLAR |
CHEBI:35696
cobalt dichloride
|
|
| 16 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.009289 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 17 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.00060072 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 18 |
Aluminum potassium sulfate
Al.K.2O4S 258.206 g/mol
|
0.000581057 MILLIMOLAR |
CHEBI:86463
potassium aluminium sulfate
|
|
| 19 |
Sodium bromide
Br.Na 102.894 g/mol
|
0.242968 MILLIMOLAR |
CHEBI:63004
sodium bromide
|
|
| 20 |
Sodium sulfide
2Na.S 78.047 g/mol
|
6.40654 MILLIMOLAR |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 21 | Nickel(II) ammonium sulfate | 0.00697114 MILLIMOLAR | ||
| 22 |
Strontium chloride
2Cl.Sr 158.526 g/mol
|
0.0142524 MILLIMOLAR |
CHEBI:36383
strontium dichloride
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.886855Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 144, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:06.782970Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.705071Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007034 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.808045+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mms medium; huber et al" to "mms_medium_huber_et_al" for consistent matching |
| 2026-06-05T07:41:35.773753+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-31T04:07:03.392862+00:00 | repair_mediadb_names.py | REPAIRED_MEDIADB_TRUNCATED_NAME | Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:144's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM. |