mm_glucose_dunn_et_al

CultureMech:007041 bacterial DEFINED LIQUID
Original name: MM-Glucose; Dunn et al

Overview

External ontology
MEDIADB:150 (source link unavailable) — MM-Glucose; Dunn et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007041["mm_glucose_dunn_et_al"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007041 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Biotin["Biotin"]
    CultureMech_007041 --> ing1_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing1_Biotin -.-> CHEBI_15956
    ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007041 --> ing2_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing3_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007041 --> ing3_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing3_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing4_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007041 --> ing4_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing4_Thiamine_HCl -.-> CHEBI_49105
    ing5_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007041 --> ing5_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing5_Magnesium_sulfate -.-> CHEBI_32599
    ing6_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007041 --> ing6_Iron_III__chloride
    ing7_Sodium_Glutamate["Sodium Glutamate"]
    CultureMech_007041 --> ing7_Sodium_Glutamate
    CHEBI_64220["CHEBI:64220"]
    ing7_Sodium_Glutamate -.-> CHEBI_64220

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
10.0 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Biotin
C10H16N2O3S 244.316 g/mol
4.09316e-05 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
3 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.360425 MILLIMOLAR CHEBI:3312
calcium dichloride
4 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
1.2629 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
5 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.000331323 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
6 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.40572 MILLIMOLAR CHEBI:32599
magnesium sulfate
7 Iron(III) chloride 0.123304 MILLIMOLAR
8 Sodium Glutamate
C5H8NO4.Na 169.112 g/mol
6.50468 MILLIMOLAR CHEBI:64220
monosodium glutamate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.942301Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 150, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:33.978735Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.743920Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007041
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.849693+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MM-Glucose; Dunn et al" to "mm_glucose_dunn_et_al" for consistent matching
2026-06-05T07:41:35.262570+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:07:03.012320+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:150's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.