Overview
- External ontology
- MEDIADB:157 (source link unavailable) — Nmm with lactate
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007047["nmm_with_lactate"]
ing0__R__Lactate["(R)-Lactate"]
CultureMech_007047 --> ing0__R__Lactate
ing1_Magnesium_chloride["Magnesium chloride"]
CultureMech_007047 --> ing1_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing1_Magnesium_chloride -.-> CHEBI_86345
ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007047 --> ing2_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing3_Potassium_sulfate["Potassium sulfate"]
CultureMech_007047 --> ing3_Potassium_sulfate
CHEBI_32036["CHEBI:32036"]
ing3_Potassium_sulfate -.-> CHEBI_32036
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007047 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_007047 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Ammonium_chloride["Ammonium chloride"]
CultureMech_007047 --> ing6_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing6_Ammonium_chloride -.-> CHEBI_31206
ing7_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007047 --> ing7_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing7_Ferrous_sulfate -.-> CHEBI_75832
ing8_MOPS["MOPS"]
CultureMech_007047 --> ing8_MOPS
CHEBI_39074["CHEBI:39074"]
ing8_MOPS -.-> CHEBI_39074
ing9_Tricine["Tricine"]
CultureMech_007047 --> ing9_Tricine
CHEBI_46760["CHEBI:46760"]
ing9_Tricine -.-> CHEBI_46760
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | (R)-Lactate | 56.0 MILLIMOLAR | Cross-references: KEGG:lac-D | |
| 2 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
0.523 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.0005 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 4 |
Potassium sulfate
2K.O4S 174.259 g/mol
|
0.276 MILLIMOLAR |
CHEBI:32036
potassium sulfate
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
1.32 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
|
50.0 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 7 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
9.52 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 8 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.01 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 9 | MOPS | 40.0 MILLIMOLAR |
CHEBI:39074
MOPS
|
|
| 10 | Tricine | 4.0 MILLIMOLAR |
CHEBI:46760
tricine
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.994102Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 157, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:26.897174Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:45.778313Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007047 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.887156+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nmm with lactate" to "nmm_with_lactate" for consistent matching |
| 2026-06-05T07:41:39.978716+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-31T04:07:06.387353+00:00 | repair_mediadb_names.py | REPAIRED_MEDIADB_TRUNCATED_NAME | Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:157's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM. |