Overview
- External ontology
- MEDIADB:165 (source link unavailable) — MM-Succinate with NH4Cl; Encarnacion et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007056["mm_succinate_with_nh4cl_encarnacion_et_al"]
ing0_Succinate["Succinate"]
CultureMech_007056 --> ing0_Succinate
CHEBI_26806["CHEBI:26806"]
ing0_Succinate -.-> CHEBI_26806
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007056 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007056 --> ing2_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007056 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Ammonium_chloride["Ammonium chloride"]
CultureMech_007056 --> ing4_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing4_Ammonium_chloride -.-> CHEBI_31206
ing5_Iron_III__chloride["Iron(III) chloride"]
CultureMech_007056 --> ing5_Iron_III__chloride
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Succinate | 10.0 MILLIMOLAR |
CHEBI:26806
succinate
|
Cross-references: KEGG:succ |
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
1.49 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
1.26 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.83 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
10.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 6 | Iron(III) chloride | 0.0184 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.059075Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 165, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.822736Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007056 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.935045+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MM-Succinate with NH4Cl; Encarnacion et al" to "mm_succinate_with_nh4cl_encarnacion_et_al" for consistent matching |
| 2026-06-05T07:41:35.306084+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-31T04:07:03.057047+00:00 | repair_mediadb_names.py | REPAIRED_MEDIADB_TRUNCATED_NAME | Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:165's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM. |