modified_m1_medium_with_18_mm_lactate_pinchuk_et_al

CultureMech:007070 bacterial DEFINED LIQUID
Original name: Modified m1 medium with 18 mm lactate; pinchuk et al

Overview

External ontology
MEDIADB:179 (source link unavailable) — Modified m1 medium with 18 mm lactate; pinchuk et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007070["modified_m1_medium_with_18_mm_lactate_pinchuk_et_al"]
    ing0_Biotin["Biotin"]
    CultureMech_007070 --> ing0_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing0_Biotin -.-> CHEBI_15956
    ing1_Nicotinate["Nicotinate"]
    CultureMech_007070 --> ing1_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing1_Nicotinate -.-> CHEBI_15940
    ing2_Riboflavin["Riboflavin"]
    CultureMech_007070 --> ing2_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing2_Riboflavin -.-> CHEBI_17015
    ing3_Thiamine["Thiamine"]
    CultureMech_007070 --> ing3_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing3_Thiamine -.-> CHEBI_18385
    ing4_Folate["Folate"]
    CultureMech_007070 --> ing4_Folate
    CHEBI_27470["CHEBI:27470"]
    ing4_Folate -.-> CHEBI_27470
    ing5_4_Aminobenzoate["4-Aminobenzoate"]
    CultureMech_007070 --> ing5_4_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing5_4_Aminobenzoate -.-> CHEBI_17836
    ing6_Lipoate["Lipoate"]
    CultureMech_007070 --> ing6_Lipoate
    CHEBI_16494["CHEBI:16494"]
    ing6_Lipoate -.-> CHEBI_16494
    ing7_Pantothenate["Pantothenate"]
    CultureMech_007070 --> ing7_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing7_Pantothenate -.-> CHEBI_16454
    ing8_Lactate["Lactate"]
    CultureMech_007070 --> ing8_Lactate
    CHEBI_24996["CHEBI:24996"]
    ing8_Lactate -.-> CHEBI_24996
    ing9_Vitamin_B12["Vitamin B12"]
    CultureMech_007070 --> ing9_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing9_Vitamin_B12 -.-> CHEBI_176843
    ing10_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007070 --> ing10_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing10_Magnesium_chloride -.-> CHEBI_86345
    ing11_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007070 --> ing11_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing11_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing12_Boric_acid["Boric acid"]
    CultureMech_007070 --> ing12_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing12_Boric_acid -.-> CHEBI_33118
    ing13_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007070 --> ing13_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing13_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_007070 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007070 --> ing15_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing15_Sodium_molybdate -.-> CHEBI_75215
    ing16_Sodium_chloride["Sodium chloride"]
    CultureMech_007070 --> ing16_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing16_Sodium_chloride -.-> CHEBI_26710
    ing17_Potassium_chloride["Potassium chloride"]
    CultureMech_007070 --> ing17_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing17_Potassium_chloride -.-> CHEBI_32588
    ing18_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_007070 --> ing18_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_HCl -.-> CHEBI_30961
    ing19_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007070 --> ing19_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing19_Ammonium_chloride -.-> CHEBI_31206
    ing20_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007070 --> ing20_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing20_Ferrous_sulfate -.-> CHEBI_75832
    ing21_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007070 --> ing21_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing21_Cobalt_chloride -.-> CHEBI_35696
    ing22_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007070 --> ing22_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing22_Zinc_sulfate -.-> CHEBI_35176
    ing23_Manganese_chloride["Manganese chloride"]
    CultureMech_007070 --> ing23_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing23_Manganese_chloride -.-> CHEBI_63041
    ing24_Cupric_chloride["Cupric chloride"]
    CultureMech_007070 --> ing24_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing24_Cupric_chloride -.-> CHEBI_49553
    ing25_Sodium_selenate["Sodium selenate"]
    CultureMech_007070 --> ing25_Sodium_selenate
    CHEBI_77775["CHEBI:77775"]
    ing25_Sodium_selenate -.-> CHEBI_77775
    ing26_Aluminum_potassium_sulfate["Aluminum potassium sulfate"]
    CultureMech_007070 --> ing26_Aluminum_potassium_sulfate
    CHEBI_86463["CHEBI:86463"]
    ing26_Aluminum_potassium_sulfate -.-> CHEBI_86463
    ing27_Sodium_tungstate["Sodium tungstate"]
    CultureMech_007070 --> ing27_Sodium_tungstate
    CHEBI_63940["CHEBI:63940"]
    ing27_Sodium_tungstate -.-> CHEBI_63940
    ing28_Nickel_Sulfate["Nickel Sulfate"]
    CultureMech_007070 --> ing28_Nickel_Sulfate
    ing29_PIPES["PIPES"]
    CultureMech_007070 --> ing29_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing29_PIPES -.-> CHEBI_39033

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 Biotin
C10H16N2O3S 244.316 g/mol
8.1863e-05 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
2 Nicotinate
C6H5NO2 123.111 g/mol
0.00040614 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
3 Riboflavin
C17H20N4O6 376.369 g/mol
0.00013285 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
4 Thiamine
C12H17N4OS 265.362 g/mol
0.00016622 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
5 Folate
C19H19N7O6 441.404 g/mol
4.531e-05 MILLIMOLAR CHEBI:27470
folic acid
 Cross-references: KEGG:fol
6 4-Aminobenzoate
C7H6NO2 136.13 g/mol
0.0003645 MILLIMOLAR CHEBI:17836
4-aminobenzoate
 Cross-references: KEGG:4abz
7 Lipoate
C8H14O2S2 206.332 g/mol
0.0002423 MILLIMOLAR CHEBI:16494
lipoic acid
 Cross-references: KEGG:lipoate
8 Pantothenate
C9H16NO5 218.229 g/mol
0.00022807 MILLIMOLAR CHEBI:16454
pantothenate
 Cross-references: KEGG:pnto-R
9 Lactate
C3H5O3 89.07 g/mol
18.0 MILLIMOLAR CHEBI:24996
lactate
10 Vitamin B12 7.3781e-07 MILLIMOLAR CHEBI:176843
vitamin B12
11 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
3.0 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
12 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.0068 MILLIMOLAR CHEBI:3312
calcium dichloride
13 Boric acid
H3BO3 61.833 g/mol
0.000808669 MILLIMOLAR CHEBI:33118
boric acid
14 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
4.35 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.11196 MILLIMOLAR CHEBI:44557
nitrilotriacetic acid
16 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.00437084 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
17 Sodium chloride
Cl.Na 58.443 g/mol
30.0 MILLIMOLAR CHEBI:26710
sodium chloride
18 Potassium chloride
Cl.K 74.551 g/mol
1.34 MILLIMOLAR CHEBI:32588
potassium chloride
19 Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
0.00048626 MILLIMOLAR CHEBI:30961
pyridoxine hydrochloride
20 Ammonium chloride
Cl.H4N 53.492 g/mol
28.0 MILLIMOLAR CHEBI:31206
ammonium chloride
21 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.0107894 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
22 Cobalt chloride
2Cl.Co 129.839 g/mol
0.0114981 MILLIMOLAR CHEBI:35696
cobalt dichloride
23 Zinc sulfate
O4S.Zn 161.453 g/mol
0.0069558 MILLIMOLAR CHEBI:35176
zinc sulfate
24 Manganese chloride
Cl2Mn 125.844 g/mol
0.0044829 MILLIMOLAR CHEBI:63041
manganese(II) chloride
25 Cupric chloride
Cl2Cu 134.452 g/mol
0.00175974 MILLIMOLAR CHEBI:49553
copper(II) chloride
26 Sodium selenate
2Na.O4Se 188.936 g/mol
0.001 MILLIMOLAR CHEBI:77775
sodium selenate
27 Aluminum potassium sulfate
Al.K.2O4S 258.206 g/mol
0.000105399 MILLIMOLAR CHEBI:86463
potassium aluminium sulfate
28 Sodium tungstate
2Na.O4W 293.816 g/mol
0.000606318 MILLIMOLAR CHEBI:63940
sodium tungstate
29 Nickel Sulfate 0.0041849 MILLIMOLAR
30 PIPES 30.0 MILLIMOLAR CHEBI:39033
PIPES

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.191661Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 179, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:17.040500Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:24.393848Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:45.913069Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007070
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.032281+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified m1 medium with 18 mm lactate; pinchuk et al" to "modified_m1_medium_with_18_mm_lactate_pinchuk_et_al" for consistent matching
2026-06-05T07:41:36.835580+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:48.827279+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).