minimal_medium_tang_et_al_2009

CultureMech:007073 bacterial DEFINED LIQUID
Original name: Minimal medium (tang et al 2009)

Overview

External ontology
MEDIADB:182 (source link unavailable) — Minimal medium (tang et al 2009)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007073["minimal_medium_tang_et_al_2009"]
    ing0_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007073 --> ing0_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing0_Magnesium_chloride -.-> CHEBI_86345
    ing1_Boric_acid["Boric acid"]
    CultureMech_007073 --> ing1_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing1_Boric_acid -.-> CHEBI_33118
    ing2_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007073 --> ing2_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing2_Sodium_sulfate -.-> CHEBI_32149
    ing3_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007073 --> ing3_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing3_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing4_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007073 --> ing4_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing4_Sodium_molybdate -.-> CHEBI_75215
    ing5_Potassium_chloride["Potassium chloride"]
    CultureMech_007073 --> ing5_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing5_Potassium_chloride -.-> CHEBI_32588
    ing6_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007073 --> ing6_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing6_Ammonium_chloride -.-> CHEBI_31206
    ing7_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007073 --> ing7_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing7_Cobalt_chloride -.-> CHEBI_35696
    ing8_Manganese_chloride["Manganese chloride"]
    CultureMech_007073 --> ing8_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing8_Manganese_chloride -.-> CHEBI_63041
    ing9_Cupric_chloride["Cupric chloride"]
    CultureMech_007073 --> ing9_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing9_Cupric_chloride -.-> CHEBI_49553
    ing10_Sodium_selenite["Sodium selenite"]
    CultureMech_007073 --> ing10_Sodium_selenite
    CHEBI_48843["CHEBI:48843"]
    ing10_Sodium_selenite -.-> CHEBI_48843
    ing11_Zinc_Chloride["Zinc Chloride"]
    CultureMech_007073 --> ing11_Zinc_Chloride
    CHEBI_49976["CHEBI:49976"]
    ing11_Zinc_Chloride -.-> CHEBI_49976
    ing12_Ferrous_chloride["Ferrous chloride"]
    CultureMech_007073 --> ing12_Ferrous_chloride
    CHEBI_30812["CHEBI:30812"]
    ing12_Ferrous_chloride -.-> CHEBI_30812
    ing13_Sodium_tungstate["Sodium tungstate"]
    CultureMech_007073 --> ing13_Sodium_tungstate
    CHEBI_63940["CHEBI:63940"]
    ing13_Sodium_tungstate -.-> CHEBI_63940
    ing14_Nickel_Sulfate["Nickel Sulfate"]
    CultureMech_007073 --> ing14_Nickel_Sulfate
    ing15_PIPES["PIPES"]
    CultureMech_007073 --> ing15_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing15_PIPES -.-> CHEBI_39033
    ing16_Sodium_L_lactate["Sodium L-lactate"]
    CultureMech_007073 --> ing16_Sodium_L_lactate
    CHEBI_232798["CHEBI:232798"]
    ing16_Sodium_L_lactate -.-> CHEBI_232798

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
1.0 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
2 Boric acid
H3BO3 61.833 g/mol
0.0032346 MILLIMOLAR CHEBI:33118
boric acid
3 Sodium sulfate
2Na.O4S 142.043 g/mol
1.5 MILLIMOLAR CHEBI:32149
sodium sulfate
4 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
4.4 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
5 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.0017986 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
6 Potassium chloride
Cl.K 74.551 g/mol
1.34 MILLIMOLAR CHEBI:32588
potassium chloride
7 Ammonium chloride
Cl.H4N 53.492 g/mol
28.0 MILLIMOLAR CHEBI:31206
ammonium chloride
8 Cobalt chloride
2Cl.Co 129.839 g/mol
0.014858 MILLIMOLAR CHEBI:35696
cobalt dichloride
9 Manganese chloride
Cl2Mn 125.844 g/mol
0.0224145 MILLIMOLAR CHEBI:63041
manganese(II) chloride
10 Cupric chloride
Cl2Cu 134.452 g/mol
0.000117316 MILLIMOLAR CHEBI:49553
copper(II) chloride
11 Sodium selenite
2Na.O3Se 172.937 g/mol
0.000228102 MILLIMOLAR CHEBI:48843
disodium selenite
12 Zinc Chloride
2Cl.Zn 136.296 g/mol
0.0146718 MILLIMOLAR CHEBI:49976
zinc dichloride
13 Ferrous chloride
Cl2Fe 126.751 g/mol
0.0502992 MILLIMOLAR CHEBI:30812
iron dichloride
14 Sodium tungstate
2Na.O4W 293.816 g/mol
0.000242527 MILLIMOLAR CHEBI:63940
sodium tungstate
15 Nickel Sulfate 0.0038044 MILLIMOLAR
16 PIPES 10.0 MILLIMOLAR CHEBI:39033
PIPES
17 Sodium L-lactate
C3H5O3.Na 112.06 g/mol
30.0 MILLIMOLAR CHEBI:232798
sodium L-lactate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T07:21:44.226667Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 182, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:13.469785Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.947430Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007073
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.069016+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Minimal medium (tang et al 2009" to "minimal_medium_tang_et_al_2009" for consistent matching
2026-06-05T07:41:34.701431+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:18:15.605374+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.