Overview
- External ontology
- MEDIADB:196 (source link unavailable) — N-c- 0.4% glycerol
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007087["n_c_0_4_glycerol"]
ing0_Glycerol["Glycerol"]
CultureMech_007087 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_Potassium_sulfate["Potassium sulfate"]
CultureMech_007087 --> ing1_Potassium_sulfate
CHEBI_32036["CHEBI:32036"]
ing1_Potassium_sulfate -.-> CHEBI_32036
ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007087 --> ing2_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing3_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007087 --> ing3_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing3_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing4_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007087 --> ing4_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing4_Magnesium_sulfate -.-> CHEBI_32599
ing5_Ammonium_chloride["Ammonium chloride"]
CultureMech_007087 --> ing5_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing5_Ammonium_chloride -.-> CHEBI_31206
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
43.436 MILLIMOLAR |
CHEBI:17754
glycerol
|
Cross-references: KEGG:glyc |
| 2 |
Potassium sulfate
2K.O4S 174.259 g/mol
|
5.7386 MILLIMOLAR |
CHEBI:32036
potassium sulfate
|
|
| 3 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
77.497 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
34.5369 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.40573 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 6 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
10.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.343254Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 196, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.045272Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007087 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.172983+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "N-c- 0.4% glycerol" to "n_c_0_4_glycerol" for consistent matching |
| 2026-06-05T07:41:39.090429+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |