medium_with_met_asp_gly_arg_fang_et_al

CultureMech:007097 bacterial DEFINED LIQUID
Original name: Medium with Met, Asp, Gly, Arg; Fang et al

Overview

External ontology
MEDIADB:204 (source link unavailable) — Medium with Met, Asp, Gly, Arg; Fang et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007097["medium_with_met_asp_gly_arg_fang_et_al"]
    ing0_Glycine["Glycine"]
    CultureMech_007097 --> ing0_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing0_Glycine -.-> CHEBI_15428
    ing1_L_Aspartate["L-Aspartate"]
    CultureMech_007097 --> ing1_L_Aspartate
    CHEBI_29991["CHEBI:29991"]
    ing1_L_Aspartate -.-> CHEBI_29991
    ing2_L_Methionine["L-Methionine"]
    CultureMech_007097 --> ing2_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing2_L_Methionine -.-> CHEBI_16643
    ing3_Glycerol["Glycerol"]
    CultureMech_007097 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_Starch["Starch"]
    CultureMech_007097 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Arginine["Arginine"]
    CultureMech_007097 --> ing5_Arginine
    CHEBI_29016["CHEBI:29016"]
    ing5_Arginine -.-> CHEBI_29016
    ing6_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007097 --> ing6_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing6_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing7_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007097 --> ing7_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing7_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing8_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007097 --> ing8_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing8_Sodium_sulfate -.-> CHEBI_32149
    ing9_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007097 --> ing9_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing9_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing10_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007097 --> ing10_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing10_Magnesium_sulfate -.-> CHEBI_32599
    ing11_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007097 --> ing11_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing11_Manganese_sulfate -.-> CHEBI_86364
    ing12_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007097 --> ing12_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing12_Ammonium_chloride -.-> CHEBI_31206
    ing13_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007097 --> ing13_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing13_Ferrous_sulfate -.-> CHEBI_75832
    ing14_Ammonium_nitrate["Ammonium nitrate"]
    CultureMech_007097 --> ing14_Ammonium_nitrate
    CHEBI_63038["CHEBI:63038"]
    ing14_Ammonium_nitrate -.-> CHEBI_63038

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Glycine
C2H5NO2 75.067 g/mol
5.32836 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
2 L-Aspartate
C4H6NO4 132.095 g/mol
15.0263 MILLIMOLAR CHEBI:29991
L-aspartate(1-)
 Cross-references: KEGG:asp-L
3 L-Methionine
C5H11NO2S 149.215 g/mol
2.68078 MILLIMOLAR CHEBI:16643
L-methionine
 Cross-references: KEGG:met-L
4 Glycerol
C3H8O3 92.094 g/mol
27.147 MILLIMOLAR CHEBI:17754
glycerol
 Cross-references: KEGG:glyc
5 Starch 154.32 MILLIMOLAR CHEBI:28017
starch
6 Arginine
C6H14N4O2 174.204 g/mol
5.7405 MILLIMOLAR CHEBI:29016
arginine
7 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.0090106 MILLIMOLAR CHEBI:3312
calcium dichloride
8 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
34.443 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
9 Sodium sulfate
2Na.O4S 142.043 g/mol
1.408 MILLIMOLAR CHEBI:32149
sodium sulfate
10 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
14.6965 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
11 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.16229 MILLIMOLAR CHEBI:32599
magnesium sulfate
12 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.008965 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
13 Ammonium chloride
Cl.H4N 53.492 g/mol
18.6947 MILLIMOLAR CHEBI:31206
ammonium chloride
14 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.0071929 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
15 Ammonium nitrate
H4N.NO3 80.043 g/mol
2.49837 MILLIMOLAR CHEBI:63038
ammonium nitrate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.415986Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 204, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.117862Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007097
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.250985+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium with Met, Asp, Gly, Arg; Fang et al" to "medium_with_met_asp_gly_arg_fang_et_al" for consistent matching
2026-06-05T07:41:30.380288+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:02:51.333695+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).