deshpande_medium_with_met_asp_gly_arg_fang_et_al

CultureMech:007100 bacterial DEFINED LIQUID
Original name: Deshpande medium with Met, Asp, Gly, Arg; Fang et al

Overview

External ontology
MEDIADB:207 (source link unavailable) — Deshpande medium with Met, Asp, Gly, Arg; Fang et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007100["deshpande_medium_with_met_asp_gly_arg_fang_et_al"]
    ing0_Glycine["Glycine"]
    CultureMech_007100 --> ing0_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing0_Glycine -.-> CHEBI_15428
    ing1_L_Aspartate["L-Aspartate"]
    CultureMech_007100 --> ing1_L_Aspartate
    CHEBI_29991["CHEBI:29991"]
    ing1_L_Aspartate -.-> CHEBI_29991
    ing2_L_Methionine["L-Methionine"]
    CultureMech_007100 --> ing2_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing2_L_Methionine -.-> CHEBI_16643
    ing3_Arginine["Arginine"]
    CultureMech_007100 --> ing3_Arginine
    CHEBI_29016["CHEBI:29016"]
    ing3_Arginine -.-> CHEBI_29016
    ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007100 --> ing4_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007100 --> ing5_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007100 --> ing6_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing7_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007100 --> ing7_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing7_Cobalt_chloride -.-> CHEBI_35696
    ing8_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007100 --> ing8_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing8_Zinc_sulfate -.-> CHEBI_35176
    ing9_Manganese_chloride["Manganese chloride"]
    CultureMech_007100 --> ing9_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing9_Manganese_chloride -.-> CHEBI_63041
    ing10_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007100 --> ing10_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing10_Cupric_sulfate -.-> CHEBI_23414
    ing11_Sodium_citrate["Sodium citrate"]
    CultureMech_007100 --> ing11_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing11_Sodium_citrate -.-> CHEBI_53258
    ing12_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007100 --> ing12_Iron_III__chloride
    ing13_Sodium_borate["Sodium borate"]
    CultureMech_007100 --> ing13_Sodium_borate
    ing14_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
    CultureMech_007100 --> ing14_Molybdic_acid_ammonium_salt_tetrahydrate
    CHEBI_91249["CHEBI:91249"]
    ing14_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Glycine
C2H5NO2 75.067 g/mol
5.32836 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
2 L-Aspartate
C4H6NO4 132.095 g/mol
15.0263 MILLIMOLAR CHEBI:29991
L-aspartate(1-)
 Cross-references: KEGG:asp-L
3 L-Methionine
C5H11NO2S 149.215 g/mol
2.68078 MILLIMOLAR CHEBI:16643
L-methionine
 Cross-references: KEGG:met-L
4 Arginine
C6H14N4O2 174.204 g/mol
5.7405 MILLIMOLAR CHEBI:29016
arginine
5 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.00450512 MILLIMOLAR CHEBI:3312
calcium dichloride
6 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
80.3673 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
7 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
44.0898 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
8 Cobalt chloride
2Cl.Co 129.839 g/mol
8.40583e-05 MILLIMOLAR CHEBI:35696
cobalt dichloride
9 Zinc sulfate
O4S.Zn 161.453 g/mol
0.00061931 MILLIMOLAR CHEBI:35176
zinc sulfate
10 Manganese chloride
Cl2Mn 125.844 g/mol
0.000313803 MILLIMOLAR CHEBI:63041
manganese(II) chloride
11 Cupric sulfate
Cu.O4S 159.609 g/mol
4.00481e-05 MILLIMOLAR CHEBI:23414
copper(II) sulfate
12 Sodium citrate
C6H5O7.3Na 258.069 g/mol
15.5003 MILLIMOLAR CHEBI:53258
sodium citrate
13 Iron(III) chloride 0.000123305 MILLIMOLAR
14 Sodium borate 0.000235991 MILLIMOLAR
15 Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
3.23661e-05 MILLIMOLAR CHEBI:91249
ammonium molybdate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.446258Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 207, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.135675Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007100
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.269438+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Deshpande medium with Met, Asp, Gly, Arg; Fang et al" to "deshpande_medium_with_met_asp_gly_arg_fang_et_al" for consistent matching
2026-06-05T07:40:55.741935+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:38.961203+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-08-31T04:06:13.397305+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:207's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.