spizizens_medium_trace_elements_0_2_glutamate_50_ug_ml_tryptophan_and_phenylalanine_1_glucose_1_pyruvate_nakano_et_al_n

CultureMech:007106 bacterial DEFINED LIQUID
Original name: Spizizen's medium + trace elements + 0.2% glutamate + 50 ug/mL tryptophan and phenylalanine + 1% glucose + 1% pyruvate; Nakano et al

Overview

External ontology
MEDIADB:212 (source link unavailable) — Spizizen's medium + trace elements + 0.2% glutamate + 50 ug/mL tryptophan and phenylalanine + 1% glucose + 1% pyruvate; Nakano et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007106["spizizens_medium_trace_elements_0_2_glutamate_50_ug_ml_tryptophan_a..."]
    ing0_L_Glutamate["L-Glutamate"]
    CultureMech_007106 --> ing0_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing0_L_Glutamate -.-> CHEBI_16015
    ing1_D_Glucose["D-Glucose"]
    CultureMech_007106 --> ing1_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing1_D_Glucose -.-> CHEBI_17634
    ing2_Tryptophan["Tryptophan"]
    CultureMech_007106 --> ing2_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing2_Tryptophan -.-> CHEBI_27897
    ing3_Phenylalanine["Phenylalanine"]
    CultureMech_007106 --> ing3_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing3_Phenylalanine -.-> CHEBI_17295
    ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007106 --> ing4_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007106 --> ing5_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing6_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007106 --> ing6_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing6_Sodium_molybdate -.-> CHEBI_75215
    ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007106 --> ing7_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing8_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007106 --> ing8_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing8_Magnesium_sulfate -.-> CHEBI_32599
    ing9_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007106 --> ing9_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing9_Manganese_sulfate -.-> CHEBI_86364
    ing10_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007106 --> ing10_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing10_Cobalt_chloride -.-> CHEBI_35696
    ing11_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007106 --> ing11_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing11_Ammonium_sulfate -.-> CHEBI_62946
    ing12_Cupric_chloride["Cupric chloride"]
    CultureMech_007106 --> ing12_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing12_Cupric_chloride -.-> CHEBI_49553
    ing13_Sodium_selenate["Sodium selenate"]
    CultureMech_007106 --> ing13_Sodium_selenate
    CHEBI_77775["CHEBI:77775"]
    ing13_Sodium_selenate -.-> CHEBI_77775
    ing14_Sodium_citrate["Sodium citrate"]
    CultureMech_007106 --> ing14_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing14_Sodium_citrate -.-> CHEBI_53258
    ing15_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007106 --> ing15_Iron_III__chloride
    ing16_Zinc_Chloride["Zinc Chloride"]
    CultureMech_007106 --> ing16_Zinc_Chloride
    CHEBI_49976["CHEBI:49976"]
    ing16_Zinc_Chloride -.-> CHEBI_49976
    ing17_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_007106 --> ing17_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing17_Sodium_pyruvate -.-> CHEBI_50144

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 L-Glutamate
C5H9NO4 147.13 g/mol
13.5934 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
2 D-Glucose
C6H12O6 180.156 g/mol
83.2593 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
3 Tryptophan
C11H12N2O2 204.229 g/mol
0.244822 MILLIMOLAR CHEBI:27897
tryptophan
4 Phenylalanine
C9H11NO2 165.192 g/mol
0.302682 MILLIMOLAR CHEBI:17295
L-phenylalanine
5 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.049556 MILLIMOLAR CHEBI:3312
calcium dichloride
6 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
80.3673 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
7 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.00247985 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
8 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
44.0898 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
9 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.811458 MILLIMOLAR CHEBI:32599
magnesium sulfate
10 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.0044828 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
11 Cobalt chloride
2Cl.Co 129.839 g/mol
0.0046211 MILLIMOLAR CHEBI:35696
cobalt dichloride
12 Ammonium sulfate
2H4N.O4S 132.141 g/mol
15.1355 MILLIMOLAR CHEBI:62946
ammonium sulfate
13 Cupric chloride
Cl2Cu 134.452 g/mol
0.0025222 MILLIMOLAR CHEBI:49553
copper(II) chloride
14 Sodium selenate
2Na.O4Se 188.936 g/mol
0.00248756 MILLIMOLAR CHEBI:77775
sodium selenate
15 Sodium citrate
C6H5O7.3Na 258.069 g/mol
3.4002 MILLIMOLAR CHEBI:53258
sodium citrate
16 Iron(III) chloride 0.04994 MILLIMOLAR
17 Zinc Chloride
2Cl.Zn 136.296 g/mol
0.012471 MILLIMOLAR CHEBI:49976
zinc dichloride
18 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
100.0 MILLIMOLAR CHEBI:50144
sodium pyruvate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.515889Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 212, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.179493Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007106
2026-03-17T06:30:19.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.317469+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Spizizen's medium + trace elements + 0.2% glutamate + 50 ug/mL tryptophan and phenylalanine + 1% glucose + 1% pyruvate; Nakano et al','N" to "spizizens_medium_trace_elements_0_2_glutamate_50_ug_ml_tryptophan_and_phenylalanine_1_glucose_1_pyruvate_nakano_et_al_n" for consistent matching
2026-06-05T07:41:46.461773+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:07:11.176708+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:212's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.
2026-08-31T06:27:07.167684+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.