Overview
- External ontology
- MEDIADB:220 (source link unavailable) — Fry et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007115["fry_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007115 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Tryptophan["Tryptophan"]
CultureMech_007115 --> ing1_Tryptophan
CHEBI_27897["CHEBI:27897"]
ing1_Tryptophan -.-> CHEBI_27897
ing2_Magnesium_chloride["Magnesium chloride"]
CultureMech_007115 --> ing2_Magnesium_chloride
CHEBI_86345["CHEBI:86345"]
ing2_Magnesium_chloride -.-> CHEBI_86345
ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007115 --> ing3_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007115 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Sodium_sulfate["Sodium sulfate"]
CultureMech_007115 --> ing5_Sodium_sulfate
CHEBI_32149["CHEBI:32149"]
ing5_Sodium_sulfate -.-> CHEBI_32149
ing6_Potassium_chloride["Potassium chloride"]
CultureMech_007115 --> ing6_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing6_Potassium_chloride -.-> CHEBI_32588
ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007115 --> ing7_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing8_Ammonium_chloride["Ammonium chloride"]
CultureMech_007115 --> ing8_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing8_Ammonium_chloride -.-> CHEBI_31206
ing9_Manganese_chloride["Manganese chloride"]
CultureMech_007115 --> ing9_Manganese_chloride
CHEBI_63041["CHEBI:63041"]
ing9_Manganese_chloride -.-> CHEBI_63041
ing10_Iron_III__chloride["Iron(III) chloride"]
CultureMech_007115 --> ing10_Iron_III__chloride
ing11_Sodium_nitrate["Sodium nitrate"]
CultureMech_007115 --> ing11_Sodium_nitrate
CHEBI_63005["CHEBI:63005"]
ing11_Sodium_nitrate -.-> CHEBI_63005
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
44.405 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Tryptophan
C11H12N2O2 204.229 g/mol
|
0.195858 MILLIMOLAR |
CHEBI:27897
tryptophan
|
|
| 3 |
Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
|
0.122965 MILLIMOLAR |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.100409 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
15.6142 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium sulfate
2Na.O4S 142.043 g/mol
|
1.99944 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
|
| 7 |
Potassium chloride
Cl.K 74.551 g/mol
|
2.01204 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 8 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
7.34829 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
18.6947 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 10 |
Manganese chloride
Cl2Mn 125.844 g/mol
|
0.0672435 MILLIMOLAR |
CHEBI:63041
manganese(II) chloride
|
|
| 11 | Iron(III) chloride | 0.00799112 MILLIMOLAR | ||
| 12 |
Sodium nitrate
NO3.Na 84.994 g/mol
|
2.00012 MILLIMOLAR |
CHEBI:63005
sodium nitrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.624602Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 220, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:28.065072Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:46.275169Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007115 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.421012+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Fry et al" to "fry_et_al" for consistent matching |
| 2026-06-05T07:41:05.580883+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-31T04:06:20.731804+00:00 | repair_mediadb_names.py | REPAIRED_MEDIADB_TRUNCATED_NAME | Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:220's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM. |