gotz_minimal_medium_with_malate

CultureMech:007119 bacterial DEFINED LIQUID
Original name: Gotz minimal medium with malate

Overview

External ontology
MEDIADB:224 (source link unavailable) — Gotz minimal medium with malate
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007119["gotz_minimal_medium_with_malate"]
    ing0_Biotin["Biotin"]
    CultureMech_007119 --> ing0_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing0_Biotin -.-> CHEBI_15956
    ing1__S__Malate["(S)-Malate"]
    CultureMech_007119 --> ing1__S__Malate
    ing2_Riboflavin["Riboflavin"]
    CultureMech_007119 --> ing2_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing2_Riboflavin -.-> CHEBI_17015
    ing3_Pyridoxine["Pyridoxine"]
    CultureMech_007119 --> ing3_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing3_Pyridoxine -.-> CHEBI_16709
    ing4_4_Aminobenzoate["4-Aminobenzoate"]
    CultureMech_007119 --> ing4_4_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing4_4_Aminobenzoate -.-> CHEBI_17836
    ing5_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007119 --> ing5_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing5_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing6_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007119 --> ing6_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing6_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing7_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007119 --> ing7_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing7_Sodium_molybdate -.-> CHEBI_75215
    ing8_Sodium_chloride["Sodium chloride"]
    CultureMech_007119 --> ing8_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing8_Sodium_chloride -.-> CHEBI_26710
    ing9_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007119 --> ing9_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing9_Thiamine_HCl -.-> CHEBI_49105
    ing10_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007119 --> ing10_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing10_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing11_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007119 --> ing11_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing11_Magnesium_sulfate -.-> CHEBI_32599
    ing12_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007119 --> ing12_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing12_Ferrous_sulfate -.-> CHEBI_75832
    ing13_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007119 --> ing13_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing13_Ammonium_sulfate -.-> CHEBI_62946
    ing14_MOPS["MOPS"]
    CultureMech_007119 --> ing14_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing14_MOPS -.-> CHEBI_39074

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Biotin
C10H16N2O3S 244.316 g/mol
8.2e-05 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
2 (S)-Malate 27.0 MILLIMOLAR Cross-references: KEGG:mal-L
3 Riboflavin
C17H20N4O6 376.369 g/mol
5.3e-05 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
4 Pyridoxine
C8H11NO3 169.18 g/mol
0.00012 MILLIMOLAR CHEBI:16709
pyridoxine
 Cross-references: KEGG:pydxn
5 4-Aminobenzoate
C7H6NO2 136.13 g/mol
0.00015 MILLIMOLAR CHEBI:17836
4-aminobenzoate
 Cross-references: KEGG:4abz
6 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.1 MILLIMOLAR CHEBI:3312
calcium dichloride
7 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
6.1 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
8 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.01 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
9 Sodium chloride
Cl.Na 58.443 g/mol
0.1 MILLIMOLAR CHEBI:26710
sodium chloride
10 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
6.6e-05 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
11 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
3.9 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
12 Magnesium sulfate
Mg.O4S 120.368 g/mol
1.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
13 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.001 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
14 Ammonium sulfate
2H4N.O4S 132.141 g/mol
1.0 MILLIMOLAR CHEBI:62946
ammonium sulfate
15 MOPS 40.0 MILLIMOLAR CHEBI:39074
MOPS

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.660707Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 224, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.298609Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007119
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.447373+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Gotz minimal medium with malate" to "gotz_minimal_medium_with_malate" for consistent matching
2026-06-05T07:41:06.624134+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:06:21.490241+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:224's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.