minimal_medium_frustaci

CultureMech:007120 bacterial DEFINED LIQUID
Original name: Minimal medium; frustaci

Overview

External ontology
MEDIADB:225 (source link unavailable) — Minimal medium; frustaci
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007120["minimal_medium_frustaci"]
    ing0_Glycerol["Glycerol"]
    CultureMech_007120 --> ing0_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing0_Glycerol -.-> CHEBI_17754
    ing1_Biotin["Biotin"]
    CultureMech_007120 --> ing1_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing1_Biotin -.-> CHEBI_15956
    ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007120 --> ing2_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing3_Boric_acid["Boric acid"]
    CultureMech_007120 --> ing3_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing3_Boric_acid -.-> CHEBI_33118
    ing4_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007120 --> ing4_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing4_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing5_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007120 --> ing5_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing5_Sodium_molybdate -.-> CHEBI_75215
    ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007120 --> ing6_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing7_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007120 --> ing7_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing7_Magnesium_sulfate -.-> CHEBI_32599
    ing8_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007120 --> ing8_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing8_Ammonium_chloride -.-> CHEBI_31206
    ing9_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007120 --> ing9_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing9_Zinc_sulfate -.-> CHEBI_35176
    ing10_Manganese_chloride["Manganese chloride"]
    CultureMech_007120 --> ing10_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing10_Manganese_chloride -.-> CHEBI_63041
    ing11_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007120 --> ing11_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing11_Cupric_sulfate -.-> CHEBI_23414
    ing12_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007120 --> ing12_Iron_III__chloride

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Glycerol
C3H8O3 92.094 g/mol
43.0 MILLIMOLAR CHEBI:17754
glycerol
 Cross-references: KEGG:glyc
2 Biotin
C10H16N2O3S 244.316 g/mol
0.00041 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
3 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.34 MILLIMOLAR CHEBI:3312
calcium dichloride
4 Boric acid
H3BO3 61.833 g/mol
0.16 MILLIMOLAR CHEBI:33118
boric acid
5 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
2.1 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
6 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.0024 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
7 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
1.7 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
8 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.41 MILLIMOLAR CHEBI:32599
magnesium sulfate
9 Ammonium chloride
Cl.H4N 53.492 g/mol
4.7 MILLIMOLAR CHEBI:31206
ammonium chloride
10 Zinc sulfate
O4S.Zn 161.453 g/mol
0.0064 MILLIMOLAR CHEBI:35176
zinc sulfate
11 Manganese chloride
Cl2Mn 125.844 g/mol
0.0025 MILLIMOLAR CHEBI:63041
manganese(II) chloride
12 Cupric sulfate
Cu.O4S 159.609 g/mol
0.002 MILLIMOLAR CHEBI:23414
copper(II) sulfate
13 Iron(III) chloride 0.0037 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.669272Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 225, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.305060Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007120
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.454372+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Minimal medium; frustaci" to "minimal_medium_frustaci" for consistent matching
2026-06-05T07:41:34.677124+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:07:02.594708+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:225's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.