glucose_salt_medium_k

CultureMech:007123 bacterial DEFINED LIQUID
Original name: Glucose salt (Medium K); Schaechter et al

Overview

External ontology
MEDIADB:228 (source link unavailable) — Glucose salt (Medium K); Schaechter et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007123["glucose_salt_medium_k"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007123 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Citrate["Citrate"]
    CultureMech_007123 --> ing1_Citrate
    CHEBI_16947["CHEBI:16947"]
    ing1_Citrate -.-> CHEBI_16947
    ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007123 --> ing2_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing3_Potassium_chloride["Potassium chloride"]
    CultureMech_007123 --> ing3_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing3_Potassium_chloride -.-> CHEBI_32588
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007123 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Sodium_ammonium_phosphate["Sodium ammonium phosphate"]
    CultureMech_007123 --> ing5_Sodium_ammonium_phosphate

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
11.1012 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Citrate
C6H5O7 189.099 g/mol
5.20497 MILLIMOLAR CHEBI:16947
citrate(3-)
 Cross-references: KEGG:cit
3 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
28.0899 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
4 Potassium chloride
Cl.K 74.551 g/mol
9.92605 MILLIMOLAR CHEBI:32588
potassium chloride
5 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.405729 MILLIMOLAR CHEBI:32599
magnesium sulfate
6 Sodium ammonium phosphate 8.32187 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.694392Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 228, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:33.758097Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:46.322304Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007123
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.473059+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Glucose salt (Medium K" to "glucose_salt_medium_k" for consistent matching
2026-06-05T07:41:06.492745+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:17:32.875689+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.