Overview
- External ontology
- MEDIADB:230 (source link unavailable) — Lactate salt; schaechter et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007126["lactate_salt_schaechter_et_al"]
ing0_Citrate["Citrate"]
CultureMech_007126 --> ing0_Citrate
CHEBI_16947["CHEBI:16947"]
ing0_Citrate -.-> CHEBI_16947
ing1__R__Lactate["(R)-Lactate"]
CultureMech_007126 --> ing1__R__Lactate
ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007126 --> ing2_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing3_Potassium_chloride["Potassium chloride"]
CultureMech_007126 --> ing3_Potassium_chloride
CHEBI_32588["CHEBI:32588"]
ing3_Potassium_chloride -.-> CHEBI_32588
ing4_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007126 --> ing4_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing4_Magnesium_sulfate -.-> CHEBI_32599
ing5_Sodium_ammonium_phosphate["Sodium ammonium phosphate"]
CultureMech_007126 --> ing5_Sodium_ammonium_phosphate
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Citrate
C6H5O7 189.099 g/mol
|
5.20497 MILLIMOLAR |
CHEBI:16947
citrate(3-)
|
Cross-references: KEGG:cit |
| 2 | (R)-Lactate | 22.4543 MILLIMOLAR | Cross-references: KEGG:lac-D | |
| 3 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
28.0899 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
Potassium chloride
Cl.K 74.551 g/mol
|
9.92605 MILLIMOLAR |
CHEBI:32588
potassium chloride
|
|
| 5 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.405729 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 6 | Sodium ammonium phosphate | 8.32187 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.712188Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 230, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-01-28T19:48:20.863926Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:46.338477Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007126 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.489662+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Lactate salt; schaechter et al" to "lactate_salt_schaechter_et_al" for consistent matching |
| 2026-06-05T07:41:10.664664+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-31T04:06:24.144879+00:00 | repair_mediadb_names.py | REPAIRED_MEDIADB_TRUNCATED_NAME | Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:230's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM. |