aspartate_salt_schaechter_et_al

CultureMech:007128 bacterial DEFINED LIQUID
Original name: Aspartate salt; schaechter et al

Overview

External ontology
MEDIADB:232 (source link unavailable) — Aspartate salt; schaechter et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007128["aspartate_salt_schaechter_et_al"]
    ing0_Citrate["Citrate"]
    CultureMech_007128 --> ing0_Citrate
    CHEBI_16947["CHEBI:16947"]
    ing0_Citrate -.-> CHEBI_16947
    ing1_D_Aspartate["D-Aspartate"]
    CultureMech_007128 --> ing1_D_Aspartate
    CHEBI_29994["CHEBI:29994"]
    ing1_D_Aspartate -.-> CHEBI_29994
    ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007128 --> ing2_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing3_Potassium_chloride["Potassium chloride"]
    CultureMech_007128 --> ing3_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing3_Potassium_chloride -.-> CHEBI_32588
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007128 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Sodium_ammonium_phosphate["Sodium ammonium phosphate"]
    CultureMech_007128 --> ing5_Sodium_ammonium_phosphate

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Citrate
C6H5O7 189.099 g/mol
5.20497 MILLIMOLAR CHEBI:16947
citrate(3-)
 Cross-references: KEGG:cit
2 D-Aspartate
C4H5NO4 131.087 g/mol
0.901578 MILLIMOLAR CHEBI:29994
D-aspartate(2-)
 Cross-references: KEGG:asp-D
3 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
28.0899 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
4 Potassium chloride
Cl.K 74.551 g/mol
9.92605 MILLIMOLAR CHEBI:32588
potassium chloride
5 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.405729 MILLIMOLAR CHEBI:32599
magnesium sulfate
6 Sodium ammonium phosphate 8.32187 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.729372Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 232, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:23.490661Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:46.347009Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007128
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.498817+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Aspartate salt; schaechter et al" to "aspartate_salt_schaechter_et_al" for consistent matching
2026-06-05T07:40:49.911507+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:32.665746+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).