Overview
- External ontology
- MEDIADB:251 (source link unavailable) — Calvo et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007149["calvo_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007149 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007149 --> ing1_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing1_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing2_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007149 --> ing2_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing2_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007149 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007149 --> ing4_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing4_Ammonium_sulfate -.-> CHEBI_62946
ing5_Sodium_citrate["Sodium citrate"]
CultureMech_007149 --> ing5_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Sodium_citrate -.-> CHEBI_53258
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
11.1012 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
60.2755 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
31.5976 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.405729 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
7.56773 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 6 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
1.50583 MILLIMOLAR |
CHEBI:53258
sodium citrate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:44.913454Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 251, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.454249Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007149 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.614079+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Calvo et al" to "calvo_et_al" for consistent matching |
| 2026-06-05T07:40:52.421559+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |