isolation_medium

CultureMech:007150 bacterial DEFINED LIQUID
Original name: Isolation medium

Overview

External ontology
MEDIADB:252 (source link unavailable) — Isolation medium
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007150["isolation_medium"]
    ing0_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007150 --> ing0_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing0_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing1_Boric_acid["Boric acid"]
    CultureMech_007150 --> ing1_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing1_Boric_acid -.-> CHEBI_33118
    ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007150 --> ing2_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007150 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007150 --> ing4_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing5_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007150 --> ing5_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing5_Magnesium_sulfate -.-> CHEBI_32599
    ing6_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007150 --> ing6_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing6_Manganese_sulfate -.-> CHEBI_86364
    ing7_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007150 --> ing7_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing7_Ferrous_sulfate -.-> CHEBI_75832
    ing8_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007150 --> ing8_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing8_Cobalt_chloride -.-> CHEBI_35696
    ing9_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007150 --> ing9_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing9_Zinc_sulfate -.-> CHEBI_35176
    ing10_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007150 --> ing10_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing10_Cupric_sulfate -.-> CHEBI_23414
    ing11_Ammonium_nitrate["Ammonium nitrate"]
    CultureMech_007150 --> ing11_Ammonium_nitrate
    CHEBI_63038["CHEBI:63038"]
    ing11_Ammonium_nitrate -.-> CHEBI_63038
    ing12_Molybdenum_trioxide["Molybdenum trioxide"]
    CultureMech_007150 --> ing12_Molybdenum_trioxide
    CHEBI_30627["CHEBI:30627"]
    ing12_Molybdenum_trioxide -.-> CHEBI_30627

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.068 MILLIMOLAR CHEBI:3312
calcium dichloride
2 Boric acid
H3BO3 61.833 g/mol
3.23e-06 MILLIMOLAR CHEBI:33118
boric acid
3 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
12.6 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
4 Sodium chloride
Cl.Na 58.443 g/mol
17.0 MILLIMOLAR CHEBI:26710
sodium chloride
5 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
11.0 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
6 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.81 MILLIMOLAR CHEBI:32599
magnesium sulfate
7 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
8.3e-06 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
8 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.036 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
9 Cobalt chloride
2Cl.Co 129.839 g/mol
8.41e-06 MILLIMOLAR CHEBI:35696
cobalt dichloride
10 Zinc sulfate
O4S.Zn 161.453 g/mol
5.68e-05 MILLIMOLAR CHEBI:35176
zinc sulfate
11 Cupric sulfate
Cu.O4S 159.609 g/mol
4e-06 MILLIMOLAR CHEBI:23414
copper(II) sulfate
12 Ammonium nitrate
H4N.NO3 80.043 g/mol
12.5 MILLIMOLAR CHEBI:63038
ammonium nitrate
13 Molybdenum trioxide
MoO3 143.937 g/mol
1.39e-05 MILLIMOLAR CHEBI:30627
molybdenum trioxide

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.921143Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 252, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.459611Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007150
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.620048+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Isolation medium" to "isolation_medium" for consistent matching
2026-06-05T07:41:09.612613+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.