hmm_with_2_g_l_junlon_pw_110_hobbs_et_al

CultureMech:007154 bacterial DEFINED LIQUID
Original name: HMM with 2 g/L Junlon PW 110; Hobbs et al

Overview

External ontology
MEDIADB:256 (source link unavailable) — HMM with 2 g/L Junlon PW 110; Hobbs et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007154["hmm_with_2_g_l_junlon_pw_110_hobbs_et_al"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007154 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Propenoate["Propenoate"]
    CultureMech_007154 --> ing1_Propenoate
    ing2_Tromethamine["Tromethamine"]
    CultureMech_007154 --> ing2_Tromethamine
    CHEBI_9754["CHEBI:9754"]
    ing2_Tromethamine -.-> CHEBI_9754
    ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007154 --> ing3_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing4_Boric_acid["Boric acid"]
    CultureMech_007154 --> ing4_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing4_Boric_acid -.-> CHEBI_33118
    ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007154 --> ing5_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing6_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007154 --> ing6_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing6_Sodium_sulfate -.-> CHEBI_32149
    ing7_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007154 --> ing7_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing7_Sodium_molybdate -.-> CHEBI_75215
    ing8_Sodium_chloride["Sodium chloride"]
    CultureMech_007154 --> ing8_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing8_Sodium_chloride -.-> CHEBI_26710
    ing9_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007154 --> ing9_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing9_Magnesium_sulfate -.-> CHEBI_32599
    ing10_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007154 --> ing10_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing10_Zinc_sulfate -.-> CHEBI_35176
    ing11_Manganese_chloride["Manganese chloride"]
    CultureMech_007154 --> ing11_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing11_Manganese_chloride -.-> CHEBI_63041
    ing12_Cupric_chloride["Cupric chloride"]
    CultureMech_007154 --> ing12_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing12_Cupric_chloride -.-> CHEBI_49553
    ing13_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007154 --> ing13_Iron_III__chloride
    ing14_Zinc_Chloride["Zinc Chloride"]
    CultureMech_007154 --> ing14_Zinc_Chloride
    CHEBI_49976["CHEBI:49976"]
    ing14_Zinc_Chloride -.-> CHEBI_49976
    ing15_Sodium_iodide["Sodium iodide"]
    CultureMech_007154 --> ing15_Sodium_iodide
    CHEBI_33167["CHEBI:33167"]
    ing15_Sodium_iodide -.-> CHEBI_33167
    ing16_Sodium_nitrate["Sodium nitrate"]
    CultureMech_007154 --> ing16_Sodium_nitrate
    CHEBI_63005["CHEBI:63005"]
    ing16_Sodium_nitrate -.-> CHEBI_63005

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
11.1012 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Propenoate 27.7546 MILLIMOLAR Cross-references: KEGG:acryl
3 Tromethamine
C4H11NO3 121.136 g/mol
9.90589 MILLIMOLAR CHEBI:9754
tris
4 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.00108626 MILLIMOLAR CHEBI:3312
calcium dichloride
5 Boric acid
H3BO3 61.833 g/mol
0.00501375 MILLIMOLAR CHEBI:33118
boric acid
6 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
11.4811 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
7 Sodium sulfate
2Na.O4S 142.043 g/mol
35.2014 MILLIMOLAR CHEBI:32149
sodium sulfate
8 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.0010002 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
9 Sodium chloride
Cl.Na 58.443 g/mol
85.5578 MILLIMOLAR CHEBI:26710
sodium chloride
10 Magnesium sulfate
Mg.O4S 120.368 g/mol
4.05729 MILLIMOLAR CHEBI:32599
magnesium sulfate
11 Zinc sulfate
O4S.Zn 161.453 g/mol
0.061931 MILLIMOLAR CHEBI:35176
zinc sulfate
12 Manganese chloride
Cl2Mn 125.844 g/mol
0.00455014 MILLIMOLAR CHEBI:63041
manganese(II) chloride
13 Cupric chloride
Cl2Cu 134.452 g/mol
0.00249296 MILLIMOLAR CHEBI:49553
copper(II) chloride
14 Iron(III) chloride 0.0540999 MILLIMOLAR
15 Zinc Chloride
2Cl.Zn 136.296 g/mol
0.0149653 MILLIMOLAR CHEBI:49976
zinc dichloride
16 Sodium iodide
I.Na 149.894 g/mol
0.0027687 MILLIMOLAR CHEBI:33167
sodium iodide
17 Sodium nitrate
NO3.Na 84.994 g/mol
52.9445 MILLIMOLAR CHEBI:63005
sodium nitrate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:44.965662Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 256, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.487980Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007154
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.650270+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HMM with 2 g/L Junlon PW 110; Hobbs et al" to "hmm_with_2_g_l_junlon_pw_110_hobbs_et_al" for consistent matching
2026-06-05T07:41:08.648172+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:51.853409+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-08-31T04:06:22.662541+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:256's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.