liquid_ma_minimal_media_succinate_urea_ammonia

CultureMech:007158 bacterial DEFINED LIQUID
Original name: Liquid MA minimal media + Succinate + Urea - Ammonia

Overview

External ontology
MEDIADB:25 (source link unavailable) — Liquid MA minimal media + Succinate + Urea - Ammonia
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007158["liquid_ma_minimal_media_succinate_urea_ammonia"]
    ing0_Succinate["Succinate"]
    CultureMech_007158 --> ing0_Succinate
    CHEBI_26806["CHEBI:26806"]
    ing0_Succinate -.-> CHEBI_26806
    ing1_Urea["Urea"]
    CultureMech_007158 --> ing1_Urea
    CHEBI_16199["CHEBI:16199"]
    ing1_Urea -.-> CHEBI_16199
    ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007158 --> ing2_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing3_Boric_acid["Boric acid"]
    CultureMech_007158 --> ing3_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing3_Boric_acid -.-> CHEBI_33118
    ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007158 --> ing4_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing5_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007158 --> ing5_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing5_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_007158 --> ing6_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing7_Nickel_chloride["Nickel chloride"]
    CultureMech_007158 --> ing7_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing7_Nickel_chloride -.-> CHEBI_34887
    ing8_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007158 --> ing8_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing8_Sodium_molybdate -.-> CHEBI_75215
    ing9_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007158 --> ing9_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing9_Magnesium_sulfate -.-> CHEBI_32599
    ing10_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007158 --> ing10_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing10_Cobalt_chloride -.-> CHEBI_35696
    ing11_Manganese_chloride["Manganese chloride"]
    CultureMech_007158 --> ing11_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing11_Manganese_chloride -.-> CHEBI_63041
    ing12_Cupric_chloride["Cupric chloride"]
    CultureMech_007158 --> ing12_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing12_Cupric_chloride -.-> CHEBI_49553
    ing13_Sodium_selenite["Sodium selenite"]
    CultureMech_007158 --> ing13_Sodium_selenite
    CHEBI_48843["CHEBI:48843"]
    ing13_Sodium_selenite -.-> CHEBI_48843
    ing14_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007158 --> ing14_Iron_III__chloride
    ing15_Zinc_Chloride["Zinc Chloride"]
    CultureMech_007158 --> ing15_Zinc_Chloride
    CHEBI_49976["CHEBI:49976"]
    ing15_Zinc_Chloride -.-> CHEBI_49976
    ing16_Chromium_III__Chloride["Chromium(III) Chloride"]
    CultureMech_007158 --> ing16_Chromium_III__Chloride

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Succinate 25.0 MILLIMOLAR CHEBI:26806
succinate
 Cross-references: KEGG:succ
2 Urea
CH4N2O 60.056 g/mol
20.0 MILLIMOLAR CHEBI:16199
urea
 Cross-references: KEGG:urea
3 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.45 MILLIMOLAR CHEBI:3312
calcium dichloride
4 Boric acid
H3BO3 61.833 g/mol
0.0003 MILLIMOLAR CHEBI:33118
boric acid
5 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
25.0 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
6 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
31.0 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
7 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
0.041 MILLIMOLAR CHEBI:44557
nitrilotriacetic acid
8 Nickel chloride
Cl2Ni 129.599 g/mol
0.0003 MILLIMOLAR CHEBI:34887
nickel dichloride
9 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.0003 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
10 Magnesium sulfate
Mg.O4S 120.368 g/mol
2.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
11 Cobalt chloride
2Cl.Co 129.839 g/mol
0.0003 MILLIMOLAR CHEBI:35696
cobalt dichloride
12 Manganese chloride
Cl2Mn 125.844 g/mol
0.001 MILLIMOLAR CHEBI:63041
manganese(II) chloride
13 Cupric chloride
Cl2Cu 134.452 g/mol
0.0003 MILLIMOLAR CHEBI:49553
copper(II) chloride
14 Sodium selenite
2Na.O3Se 172.937 g/mol
0.0003 MILLIMOLAR CHEBI:48843
disodium selenite
15 Iron(III) chloride 0.003 MILLIMOLAR
16 Zinc Chloride
2Cl.Zn 136.296 g/mol
0.001 MILLIMOLAR CHEBI:49976
zinc dichloride
17 Chromium(III) Chloride 0.0003 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:42.732457Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 25, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.510740Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007158
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.674166+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Liquid MA minimal media + Succinate + Urea - Ammonia" to "liquid_ma_minimal_media_succinate_urea_ammonia" for consistent matching
2026-06-05T07:41:11.301791+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:06:24.701830+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 2 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:25's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.