liquid_mm_with_glycerol_hodgson_et_al

CultureMech:007163 bacterial DEFINED LIQUID
Original name: Liquid mm with glycerol; hodgson et al

Overview

External ontology
MEDIADB:264 (source link unavailable) — Liquid mm with glycerol; hodgson et al
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007163["liquid_mm_with_glycerol_hodgson_et_al"]
    ing0_Glycerol["Glycerol"]
    CultureMech_007163 --> ing0_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing0_Glycerol -.-> CHEBI_17754
    ing1_L_Asparagine["L-Asparagine"]
    CultureMech_007163 --> ing1_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing1_L_Asparagine -.-> CHEBI_17196
    ing2_Ethylene_glycol["Ethylene glycol"]
    CultureMech_007163 --> ing2_Ethylene_glycol
    CHEBI_30742["CHEBI:30742"]
    ing2_Ethylene_glycol -.-> CHEBI_30742
    ing3_Potassium_hydroxide["Potassium hydroxide"]
    CultureMech_007163 --> ing3_Potassium_hydroxide
    CHEBI_32035["CHEBI:32035"]
    ing3_Potassium_hydroxide -.-> CHEBI_32035
    ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007163 --> ing4_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing5_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007163 --> ing5_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing5_Magnesium_sulfate -.-> CHEBI_32599
    ing6_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007163 --> ing6_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing6_Ferrous_sulfate -.-> CHEBI_75832

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Glycerol
C3H8O3 92.094 g/mol
10.0 MILLIMOLAR CHEBI:17754
glycerol
 Cross-references: KEGG:glyc
2 L-Asparagine
C4H8N2O3 132.119 g/mol
3.78444 MILLIMOLAR CHEBI:17196
L-asparagine
 Cross-references: KEGG:asn-L
3 Ethylene glycol
C2H6O2 62.068 g/mol
0.134257 MILLIMOLAR CHEBI:30742
ethylene glycol
4 Potassium hydroxide
HO.K 56.105 g/mol
5.34706 MILLIMOLAR CHEBI:32035
potassium hydroxide
5 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
2.87026 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
6 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.811458 MILLIMOLAR CHEBI:32599
magnesium sulfate
7 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.0359648 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.044341Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 264, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.533991Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007163
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.700048+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Liquid mm with glycerol; hodgson et al" to "liquid_mm_with_glycerol_hodgson_et_al" for consistent matching
2026-06-05T07:41:11.342663+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.