cgc_w_acetate_glucose

CultureMech:007185 bacterial DEFINED LIQUID
Original name: CgC w/ acetate + glucose

Overview

External ontology
MEDIADB:284 (source link unavailable) — CgC w/ acetate + glucose
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007185["cgc_w_acetate_glucose"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007185 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Acetate["Acetate"]
    CultureMech_007185 --> ing1_Acetate
    CHEBI_30089["CHEBI:30089"]
    ing1_Acetate -.-> CHEBI_30089
    ing2_Urea["Urea"]
    CultureMech_007185 --> ing2_Urea
    CHEBI_16199["CHEBI:16199"]
    ing2_Urea -.-> CHEBI_16199
    ing3_Biotin["Biotin"]
    CultureMech_007185 --> ing3_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing3_Biotin -.-> CHEBI_15956
    ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007185 --> ing4_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007185 --> ing5_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing6_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007185 --> ing6_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing6_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing7_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007185 --> ing7_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing7_Magnesium_sulfate -.-> CHEBI_32599
    ing8_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007185 --> ing8_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing8_Manganese_sulfate -.-> CHEBI_86364
    ing9_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007185 --> ing9_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing9_Ferrous_sulfate -.-> CHEBI_75832
    ing10_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007185 --> ing10_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing10_Zinc_sulfate -.-> CHEBI_35176
    ing11_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007185 --> ing11_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing11_Ammonium_sulfate -.-> CHEBI_62946
    ing12_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007185 --> ing12_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing12_Cupric_sulfate -.-> CHEBI_23414
    ing13_MOPS["MOPS"]
    CultureMech_007185 --> ing13_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing13_MOPS -.-> CHEBI_39074

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
55.0 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Acetate
C2H3O2 59.044 g/mol
120.0 MILLIMOLAR CHEBI:30089
acetate
 Cross-references: KEGG:ac
3 Urea
CH4N2O 60.056 g/mol
83.3 MILLIMOLAR CHEBI:16199
urea
 Cross-references: KEGG:urea
4 Biotin
C10H16N2O3S 244.316 g/mol
0.000819 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
5 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.0775 MILLIMOLAR CHEBI:3312
calcium dichloride
6 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
2.87 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
7 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
3.67 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
8 Magnesium sulfate
Mg.O4S 120.368 g/mol
1.01 MILLIMOLAR CHEBI:32599
magnesium sulfate
9 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.0592 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
10 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.036 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
11 Zinc sulfate
O4S.Zn 161.453 g/mol
0.00348 MILLIMOLAR CHEBI:35176
zinc sulfate
12 Ammonium sulfate
2H4N.O4S 132.141 g/mol
37.8 MILLIMOLAR CHEBI:62946
ammonium sulfate
13 Cupric sulfate
Cu.O4S 159.609 g/mol
0.00125 MILLIMOLAR CHEBI:23414
copper(II) sulfate
14 MOPS 100.0 MILLIMOLAR CHEBI:39074
MOPS

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.231241Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 284, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.662787Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007185
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.840862+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CgC w/ acetate + glucose" to "cgc_w_acetate_glucose" for consistent matching
2026-06-05T07:40:53.002035+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.