Overview
- External ontology
- MEDIADB:285 (source link unavailable) — CgC w/ glucose
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007186["cgc_w_glucose"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007186 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Urea["Urea"]
CultureMech_007186 --> ing1_Urea
CHEBI_16199["CHEBI:16199"]
ing1_Urea -.-> CHEBI_16199
ing2_Biotin["Biotin"]
CultureMech_007186 --> ing2_Biotin
CHEBI_15956["CHEBI:15956"]
ing2_Biotin -.-> CHEBI_15956
ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007186 --> ing3_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007186 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007186 --> ing5_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing6_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007186 --> ing6_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing6_Magnesium_sulfate -.-> CHEBI_32599
ing7_Manganese_sulfate["Manganese sulfate"]
CultureMech_007186 --> ing7_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing7_Manganese_sulfate -.-> CHEBI_86364
ing8_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007186 --> ing8_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing8_Ferrous_sulfate -.-> CHEBI_75832
ing9_Zinc_sulfate["Zinc sulfate"]
CultureMech_007186 --> ing9_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing9_Zinc_sulfate -.-> CHEBI_35176
ing10_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007186 --> ing10_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing10_Ammonium_sulfate -.-> CHEBI_62946
ing11_Cupric_sulfate["Cupric sulfate"]
CultureMech_007186 --> ing11_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing11_Cupric_sulfate -.-> CHEBI_23414
ing12_MOPS["MOPS"]
CultureMech_007186 --> ing12_MOPS
CHEBI_39074["CHEBI:39074"]
ing12_MOPS -.-> CHEBI_39074
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
110.0 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Urea
CH4N2O 60.056 g/mol
|
83.3 MILLIMOLAR |
CHEBI:16199
urea
|
Cross-references: KEGG:urea |
| 3 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.000819 MILLIMOLAR |
CHEBI:15956
biotin
|
Cross-references: KEGG:btn |
| 4 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.0775 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
2.87 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
3.67 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
1.01 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.0592 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 9 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.036 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 10 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.00348 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 11 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
37.8 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
|
| 12 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.00125 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 13 | MOPS | 100.0 MILLIMOLAR |
CHEBI:39074
MOPS
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.239860Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 285, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.669297Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007186 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.848215+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CgC w/ glucose" to "cgc_w_glucose" for consistent matching |
| 2026-06-05T07:40:53.010558+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |