cmm

CultureMech:007191 bacterial DEFINED LIQUID
Original name: CMM

Overview

External ontology
MEDIADB:290 (source link unavailable) — CMM
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007191["cmm"]
    ing0_Pyruvate["Pyruvate"]
    CultureMech_007191 --> ing0_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing0_Pyruvate -.-> CHEBI_15361
    ing1_L_Glutamate["L-Glutamate"]
    CultureMech_007191 --> ing1_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing1_L_Glutamate -.-> CHEBI_16015
    ing2_Glycine["Glycine"]
    CultureMech_007191 --> ing2_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing2_Glycine -.-> CHEBI_15428
    ing3_L_Alanine["L-Alanine"]
    CultureMech_007191 --> ing3_L_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing3_L_Alanine -.-> CHEBI_16977
    ing4_L_Aspartate["L-Aspartate"]
    CultureMech_007191 --> ing4_L_Aspartate
    CHEBI_29991["CHEBI:29991"]
    ing4_L_Aspartate -.-> CHEBI_29991
    ing5_L_Arginine["L-Arginine"]
    CultureMech_007191 --> ing5_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing5_L_Arginine -.-> CHEBI_16467
    ing6_L_Serine["L-Serine"]
    CultureMech_007191 --> ing6_L_Serine
    CHEBI_17115["CHEBI:17115"]
    ing6_L_Serine -.-> CHEBI_17115
    ing7_L_Phenylalanine["L-Phenylalanine"]
    CultureMech_007191 --> ing7_L_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing7_L_Phenylalanine -.-> CHEBI_17295
    ing8_Glycerol["Glycerol"]
    CultureMech_007191 --> ing8_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing8_Glycerol -.-> CHEBI_17754
    ing9_Biotin["Biotin"]
    CultureMech_007191 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_L_Leucine["L-Leucine"]
    CultureMech_007191 --> ing10_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing10_L_Leucine -.-> CHEBI_15603
    ing11_L_Asparagine["L-Asparagine"]
    CultureMech_007191 --> ing11_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing11_L_Asparagine -.-> CHEBI_17196
    ing12_L_Isoleucine["L-Isoleucine"]
    CultureMech_007191 --> ing12_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing12_L_Isoleucine -.-> CHEBI_17191
    ing13_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007191 --> ing13_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing13_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing14_Tween_80["Tween 80"]
    CultureMech_007191 --> ing14_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing14_Tween_80 -.-> CHEBI_53426
    ing15_Potassium_hydroxide["Potassium hydroxide"]
    CultureMech_007191 --> ing15_Potassium_hydroxide
    CHEBI_32035["CHEBI:32035"]
    ing15_Potassium_hydroxide -.-> CHEBI_32035
    ing16_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_007191 --> ing16_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing16_Sodium_bicarbonate -.-> CHEBI_32139
    ing17_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007191 --> ing17_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing17_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing18_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007191 --> ing18_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing18_Magnesium_sulfate -.-> CHEBI_32599
    ing19_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007191 --> ing19_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing19_Ferrous_sulfate -.-> CHEBI_75832
    ing20_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007191 --> ing20_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing20_Cobalt_chloride -.-> CHEBI_35696
    ing21_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007191 --> ing21_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing21_Zinc_sulfate -.-> CHEBI_35176
    ing22_Manganese_chloride["Manganese chloride"]
    CultureMech_007191 --> ing22_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing22_Manganese_chloride -.-> CHEBI_63041
    ing23_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007191 --> ing23_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing23_Cupric_sulfate -.-> CHEBI_23414
    ing24_ACES["ACES"]
    CultureMech_007191 --> ing24_ACES
    CHEBI_39061["CHEBI:39061"]
    ing24_ACES -.-> CHEBI_39061

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 Pyruvate
C3H3O3 87.054 g/mol
9.09 MILLIMOLAR CHEBI:15361
pyruvate
 Cross-references: KEGG:pyr
2 L-Glutamate
C5H9NO4 147.13 g/mol
0.68 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
3 Glycine
C2H5NO2 75.067 g/mol
1.33 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
4 L-Alanine
C3H7NO2 89.094 g/mol
1.12 MILLIMOLAR CHEBI:16977
L-alanine
 Cross-references: KEGG:ala-L
5 L-Aspartate
C4H6NO4 132.095 g/mol
0.751 MILLIMOLAR CHEBI:29991
L-aspartate(1-)
 Cross-references: KEGG:asp-L
6 L-Arginine
C6H14N4O2 174.204 g/mol
0.574 MILLIMOLAR CHEBI:16467
L-arginine
 Cross-references: KEGG:arg-L
7 L-Serine
C3H7NO3 105.093 g/mol
0.952 MILLIMOLAR CHEBI:17115
L-serine
 Cross-references: KEGG:ser-L
8 L-Phenylalanine
C9H11NO2 165.192 g/mol
0.605 MILLIMOLAR CHEBI:17295
L-phenylalanine
 Cross-references: KEGG:phe-L
9 Glycerol
C3H8O3 92.094 g/mol
27.4 MILLIMOLAR CHEBI:17754
glycerol
 Cross-references: KEGG:glyc
10 Biotin
C10H16N2O3S 244.316 g/mol
0.000409 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
11 L-Leucine
C6H13NO2 131.175 g/mol
0.762 MILLIMOLAR CHEBI:15603
L-leucine
 Cross-references: KEGG:leu-L
12 L-Asparagine
C4H8N2O3 132.119 g/mol
15.1 MILLIMOLAR CHEBI:17196
L-asparagine
 Cross-references: KEGG:asn-L
13 L-Isoleucine
C6H13NO2 131.175 g/mol
0.762 MILLIMOLAR CHEBI:17191
L-isoleucine
 Cross-references: KEGG:ile-L
14 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.00374 MILLIMOLAR CHEBI:3312
calcium dichloride
15 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1.53 MILLIMOLAR CHEBI:53426
polysorbate 80
16 Potassium hydroxide
HO.K 56.105 g/mol
1.0 MILLIMOLAR CHEBI:32035
potassium hydroxide
17 Sodium bicarbonate
CHO3.Na 84.006 g/mol
0.5 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
18 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
1.63 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
19 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.8869 MILLIMOLAR CHEBI:32599
magnesium sulfate
20 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.367 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
21 Cobalt chloride
2Cl.Co 129.839 g/mol
0.00202 MILLIMOLAR CHEBI:35696
cobalt dichloride
22 Zinc sulfate
O4S.Zn 161.453 g/mol
0.00141 MILLIMOLAR CHEBI:35176
zinc sulfate
23 Manganese chloride
Cl2Mn 125.844 g/mol
0.000101 MILLIMOLAR CHEBI:63041
manganese(II) chloride
24 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0001 MILLIMOLAR CHEBI:23414
copper(II) sulfate
25 ACES 54.9 MILLIMOLAR CHEBI:39061
ACES

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.272466Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 290, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:32.406433Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:46.709771Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007191
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.889442+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CMM" to "cmm" for consistent matching
2026-06-05T07:40:54.583383+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.652493+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:01:38.101290+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).