Overview
- External ontology
- MEDIADB:291 (source link unavailable) — Galani MM
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007192["galani_mm"]
ing0_Ammonium_chloride["Ammonium chloride"]
CultureMech_007192 --> ing0_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing0_Ammonium_chloride -.-> CHEBI_31206
ing1_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007192 --> ing1_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing1_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007192 --> ing2_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007192 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_007192 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Pantothenate["Pantothenate"]
CultureMech_007192 --> ing5_Pantothenate
CHEBI_16454["CHEBI:16454"]
ing5_Pantothenate -.-> CHEBI_16454
ing6_D_Glucose["D-Glucose"]
CultureMech_007192 --> ing6_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D_Glucose -.-> CHEBI_17634
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
0.019 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
|
| 2 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
0.0073 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
0.0057 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.002 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
|
0.0086 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 6 |
Pantothenate
C9H16NO5 218.229 g/mol
|
2.3e-06 MILLIMOLAR |
CHEBI:16454
pantothenate
|
Cross-references: KEGG:pnto-R |
| 7 |
D-Glucose
C6H12O6 180.156 g/mol
|
0.11 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.282302Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 291, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.716816Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007192 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:04.897120+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Galani MM" to "galani_mm" for consistent matching |
| 2026-06-05T07:41:05.885736+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |