m9_tomaras

CultureMech:007195 bacterial DEFINED LIQUID
Original name: M9 - tomaras

Overview

External ontology
MEDIADB:294 (source link unavailable) — M9 - tomaras
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007195["m9_tomaras"]
    ing0_Tromethamine["Tromethamine"]
    CultureMech_007195 --> ing0_Tromethamine
    CHEBI_9754["CHEBI:9754"]
    ing0_Tromethamine -.-> CHEBI_9754
    ing1_Potassium_chloride["Potassium chloride"]
    CultureMech_007195 --> ing1_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing1_Potassium_chloride -.-> CHEBI_32588
    ing2_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007195 --> ing2_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing2_Ammonium_chloride -.-> CHEBI_31206
    ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007195 --> ing3_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing4_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007195 --> ing4_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing4_Sodium_sulfate -.-> CHEBI_32149
    ing5_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007195 --> ing5_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing5_Magnesium_chloride -.-> CHEBI_86345
    ing6_Sodium_chloride["Sodium chloride"]
    CultureMech_007195 --> ing6_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing6_Sodium_chloride -.-> CHEBI_26710
    ing7_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007195 --> ing7_Iron_III__chloride
    ing8_EDDHA["EDDHA"]
    CultureMech_007195 --> ing8_EDDHA

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Tromethamine
C4H11NO3 121.136 g/mol
99.9 MILLIMOLAR CHEBI:9754
tris
2 Potassium chloride
Cl.K 74.551 g/mol
49.6 MILLIMOLAR CHEBI:32588
potassium chloride
3 Ammonium chloride
Cl.H4N 53.492 g/mol
20.6 MILLIMOLAR CHEBI:31206
ammonium chloride
4 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
1.02 MILLIMOLAR CHEBI:3312
calcium dichloride
5 Sodium sulfate
2Na.O4S 142.043 g/mol
1.0 MILLIMOLAR CHEBI:32149
sodium sulfate
6 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.492 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
7 Sodium chloride
Cl.Na 58.443 g/mol
85.6 MILLIMOLAR CHEBI:26710
sodium chloride
8 Iron(III) chloride 0.2 MILLIMOLAR
9 EDDHA 0.2 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.307443Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 294, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:13.539100Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:46.737938Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007195
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.919379+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M9 - tomaras" to "m9_tomaras" for consistent matching
2026-06-05T07:41:12.102527+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:06:25.348870+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:294's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.