asp2

CultureMech:007196 bacterial DEFINED LIQUID
Original name: Asp2

Overview

External ontology
MEDIADB:295 (source link unavailable) — Asp2
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007196["asp2"]
    ing0_Sodium_chloride["Sodium chloride"]
    CultureMech_007196 --> ing0_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing0_Sodium_chloride -.-> CHEBI_26710
    ing1_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007196 --> ing1_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing1_Magnesium_sulfate -.-> CHEBI_32599
    ing2_Potassium_chloride["Potassium chloride"]
    CultureMech_007196 --> ing2_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing2_Potassium_chloride -.-> CHEBI_32588
    ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007196 --> ing3_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing4_Sodium_nitrate["Sodium nitrate"]
    CultureMech_007196 --> ing4_Sodium_nitrate
    CHEBI_63005["CHEBI:63005"]
    ing4_Sodium_nitrate -.-> CHEBI_63005
    ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007196 --> ing5_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing6_Tromethamine["Tromethamine"]
    CultureMech_007196 --> ing6_Tromethamine
    CHEBI_9754["CHEBI:9754"]
    ing6_Tromethamine -.-> CHEBI_9754
    ing7_Vitamin_B12["Vitamin B12"]
    CultureMech_007196 --> ing7_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing7_Vitamin_B12 -.-> CHEBI_176843
    ing8_EDTA["EDTA"]
    CultureMech_007196 --> ing8_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing8_EDTA -.-> CHEBI_4735
    ing9_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007196 --> ing9_Iron_III__chloride
    ing10_Zinc_Chloride["Zinc Chloride"]
    CultureMech_007196 --> ing10_Zinc_Chloride
    CHEBI_49976["CHEBI:49976"]
    ing10_Zinc_Chloride -.-> CHEBI_49976
    ing11_Manganese_chloride["Manganese chloride"]
    CultureMech_007196 --> ing11_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing11_Manganese_chloride -.-> CHEBI_63041
    ing12_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007196 --> ing12_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing12_Cobalt_chloride -.-> CHEBI_35696
    ing13_Cupric_chloride["Cupric chloride"]
    CultureMech_007196 --> ing13_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing13_Cupric_chloride -.-> CHEBI_49553
    ing14_Boric_acid["Boric acid"]
    CultureMech_007196 --> ing14_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing14_Boric_acid -.-> CHEBI_33118
    ing15_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007196 --> ing15_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing15_Thiamine_HCl -.-> CHEBI_49105
    ing16_Nicotinate["Nicotinate"]
    CultureMech_007196 --> ing16_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinate -.-> CHEBI_15940
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007196 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_4_Aminobenzoate["4-Aminobenzoate"]
    CultureMech_007196 --> ing18_4_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing18_4_Aminobenzoate -.-> CHEBI_17836
    ing19_Biotin["Biotin"]
    CultureMech_007196 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_L_Inositol["L-Inositol"]
    CultureMech_007196 --> ing20_L_Inositol
    CHEBI_27374["CHEBI:27374"]
    ing20_L_Inositol -.-> CHEBI_27374
    ing21_Folate["Folate"]
    CultureMech_007196 --> ing21_Folate
    CHEBI_27470["CHEBI:27470"]
    ing21_Folate -.-> CHEBI_27470
    ing22_Thymine["Thymine"]
    CultureMech_007196 --> ing22_Thymine
    CHEBI_17821["CHEBI:17821"]
    ing22_Thymine -.-> CHEBI_17821
    ing23_sodium_silicate["sodium silicate"]
    CultureMech_007196 --> ing23_sodium_silicate
    CHEBI_60720["CHEBI:60720"]
    ing23_sodium_silicate -.-> CHEBI_60720

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 Sodium chloride
Cl.Na 58.443 g/mol
308.0 MILLIMOLAR CHEBI:26710
sodium chloride
2 Magnesium sulfate
Mg.O4S 120.368 g/mol
20.3 MILLIMOLAR CHEBI:32599
magnesium sulfate
3 Potassium chloride
Cl.K 74.551 g/mol
8.05 MILLIMOLAR CHEBI:32588
potassium chloride
4 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.901 MILLIMOLAR CHEBI:3312
calcium dichloride
5 Sodium nitrate
NO3.Na 84.994 g/mol
0.588 MILLIMOLAR CHEBI:63005
sodium nitrate
6 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
0.0287 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
7 Tromethamine
C4H11NO3 121.136 g/mol
8.25 MILLIMOLAR CHEBI:9754
tris
8 Vitamin B12 1.48e-06 MILLIMOLAR CHEBI:176843
vitamin B12
9 EDTA
C10H16N2O8 292.244 g/mol
0.131 MILLIMOLAR CHEBI:4735
ethylenediaminetetraacetic acid
10 Iron(III) chloride 0.00493 MILLIMOLAR
11 Zinc Chloride
2Cl.Zn 136.296 g/mol
0.0011 MILLIMOLAR CHEBI:49976
zinc dichloride
12 Manganese chloride
Cl2Mn 125.844 g/mol
9.54e-06 MILLIMOLAR CHEBI:63041
manganese(II) chloride
13 Cobalt chloride
2Cl.Co 129.839 g/mol
2.31e-05 MILLIMOLAR CHEBI:35696
cobalt dichloride
14 Cupric chloride
Cl2Cu 134.452 g/mol
8.93e-06 MILLIMOLAR CHEBI:49553
copper(II) chloride
15 Boric acid
H3BO3 61.833 g/mol
9.7e-05 MILLIMOLAR CHEBI:33118
boric acid
16 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.00166 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
17 Nicotinate
C6H5NO2 123.111 g/mol
0.000812 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.000456 MILLIMOLAR CHEBI:31345
Calcium pantothenate
19 4-Aminobenzoate
C7H6NO2 136.13 g/mol
7.29e-05 MILLIMOLAR CHEBI:17836
4-aminobenzoate
 Cross-references: KEGG:4abz
20 Biotin
C10H16N2O3S 244.316 g/mol
4.09e-06 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
21 L-Inositol
C6H12O6 180.156 g/mol
0.0278 MILLIMOLAR CHEBI:27374
1L-chiro-inositol
22 Folate
C19H19N7O6 441.404 g/mol
4.53e-06 MILLIMOLAR CHEBI:27470
folic acid
 Cross-references: KEGG:fol
23 Thymine
C5H6N2O2 126.115 g/mol
0.0238 MILLIMOLAR CHEBI:17821
thymine
 Cross-references: KEGG:thym
24 sodium silicate
2Na.O3Si 122.063 g/mol
1.23 MILLIMOLAR CHEBI:60720
sodium silicate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.315388Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 295, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:25.096811Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:33.837105Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:46.746731Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007196
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:04.928226+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Asp2" to "asp2" for consistent matching
2026-06-05T07:40:49.903202+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:32.656596+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-08-31T04:06:09.085365+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:295's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.