Overview
- External ontology
- MEDIADB:301 (source link unavailable) — Basal synthetic medium
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007204["basal_synthetic_medium"]
ing0_Lactose["Lactose"]
CultureMech_007204 --> ing0_Lactose
CHEBI_17716["CHEBI:17716"]
ing0_Lactose -.-> CHEBI_17716
ing1_Ammonium_phosphate["Ammonium phosphate"]
CultureMech_007204 --> ing1_Ammonium_phosphate
ing2_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007204 --> ing2_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing2_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007204 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007204 --> ing4_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing4_Ferrous_sulfate -.-> CHEBI_75832
ing5_Sodium_molybdate["Sodium molybdate"]
CultureMech_007204 --> ing5_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing5_Sodium_molybdate -.-> CHEBI_75215
ing6_Cupric_sulfate["Cupric sulfate"]
CultureMech_007204 --> ing6_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing6_Cupric_sulfate -.-> CHEBI_23414
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Lactose
C12H22O11 342.297 g/mol
|
233.7 MILLIMOLAR |
CHEBI:17716
lactose
|
Cross-references: KEGG:lcts |
| 2 | Ammonium phosphate | 121.2 MILLIMOLAR | ||
| 3 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
11.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.0812 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 5 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.072 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 6 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.00827 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 7 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.0401 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.383792Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 301, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.854599Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007204 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.037319+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Basal synthetic medium" to "basal_synthetic_medium" for consistent matching |
| 2026-06-05T07:40:50.580637+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |