basal_synthetic_medium_vitamins

CultureMech:007205 bacterial DEFINED LIQUID
Original name: Basal Synthetic Medium + vitamins

Overview

External ontology
MEDIADB:302 (source link unavailable) — Basal Synthetic Medium + vitamins
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007205["basal_synthetic_medium_vitamins"]
    ing0_4_Hydroxy_L_proline["4-Hydroxy-L-proline"]
    CultureMech_007205 --> ing0_4_Hydroxy_L_proline
    CHEBI_18240["CHEBI:18240"]
    ing0_4_Hydroxy_L_proline -.-> CHEBI_18240
    ing1_L_Proline["L-Proline"]
    CultureMech_007205 --> ing1_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing1_L_Proline -.-> CHEBI_17203
    ing2_L_Glutamine["L-Glutamine"]
    CultureMech_007205 --> ing2_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing2_L_Glutamine -.-> CHEBI_18050
    ing3_L_Glutamate["L-Glutamate"]
    CultureMech_007205 --> ing3_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing3_L_Glutamate -.-> CHEBI_16015
    ing4_L_Arginine["L-Arginine"]
    CultureMech_007205 --> ing4_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing4_L_Arginine -.-> CHEBI_16467
    ing5_L_Isoleucine["L-Isoleucine"]
    CultureMech_007205 --> ing5_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing5_L_Isoleucine -.-> CHEBI_17191
    ing6_Lactose["Lactose"]
    CultureMech_007205 --> ing6_Lactose
    CHEBI_17716["CHEBI:17716"]
    ing6_Lactose -.-> CHEBI_17716
    ing7_Ammonium_phosphate["Ammonium phosphate"]
    CultureMech_007205 --> ing7_Ammonium_phosphate
    ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007205 --> ing8_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing9_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007205 --> ing9_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing9_Magnesium_sulfate -.-> CHEBI_32599
    ing10_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007205 --> ing10_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing10_Ferrous_sulfate -.-> CHEBI_75832
    ing11_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007205 --> ing11_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing11_Sodium_molybdate -.-> CHEBI_75215
    ing12_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007205 --> ing12_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing12_Cupric_sulfate -.-> CHEBI_23414

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 4-Hydroxy-L-proline
C5H9NO3 131.131 g/mol
1.14 MILLIMOLAR CHEBI:18240
4-hydroxy-L-proline
2 L-Proline
C5H9NO2 115.132 g/mol
1.3 MILLIMOLAR CHEBI:17203
L-proline
 Cross-references: KEGG:pro-L
3 L-Glutamine
C5H10N2O3 146.146 g/mol
1.03 MILLIMOLAR CHEBI:18050
L-glutamine
 Cross-references: KEGG:gln-L
4 L-Glutamate
C5H9NO4 147.13 g/mol
1.02 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
5 L-Arginine
C6H14N4O2 174.204 g/mol
0.861 MILLIMOLAR CHEBI:16467
L-arginine
 Cross-references: KEGG:arg-L
6 L-Isoleucine
C6H13NO2 131.175 g/mol
1.14 MILLIMOLAR CHEBI:17191
L-isoleucine
 Cross-references: KEGG:ile-L
7 Lactose
C12H22O11 342.297 g/mol
233.7 MILLIMOLAR CHEBI:17716
lactose
 Cross-references: KEGG:lcts
8 Ammonium phosphate 121.2 MILLIMOLAR
9 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
11.0 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
10 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.0812 MILLIMOLAR CHEBI:32599
magnesium sulfate
11 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.072 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
12 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.00827 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
13 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0401 MILLIMOLAR CHEBI:23414
copper(II) sulfate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.392908Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 302, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.860628Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007205
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.043097+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Basal Synthetic Medium + vitamins" to "basal_synthetic_medium_vitamins" for consistent matching
2026-06-05T07:40:50.587470+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:33.888768+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).