Overview
- External ontology
- MEDIADB:312 (source link unavailable) — CDM for Batch Cultivation
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007211["cdm_for_batch_cultivation"]
ing0_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007211 --> ing0_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing0_Ferrous_sulfate -.-> CHEBI_75832
ing1_Nickel_chloride["Nickel chloride"]
CultureMech_007211 --> ing1_Nickel_chloride
CHEBI_34887["CHEBI:34887"]
ing1_Nickel_chloride -.-> CHEBI_34887
ing2_Glucose["Glucose"]
CultureMech_007211 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007211 --> ing3_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_007211 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Cupric_sulfate["Cupric sulfate"]
CultureMech_007211 --> ing5_Cupric_sulfate
CHEBI_23414["CHEBI:23414"]
ing5_Cupric_sulfate -.-> CHEBI_23414
ing6_Zinc_sulfate["Zinc sulfate"]
CultureMech_007211 --> ing6_Zinc_sulfate
CHEBI_35176["CHEBI:35176"]
ing6_Zinc_sulfate -.-> CHEBI_35176
ing7_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007211 --> ing7_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing7_Magnesium_sulfate -.-> CHEBI_32599
ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007211 --> ing8_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing9_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007211 --> ing9_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing9_Ammonium_sulfate -.-> CHEBI_62946
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.02482 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 2 |
Nickel chloride
Cl2Ni 129.599 g/mol
|
0.001052 MILLIMOLAR |
CHEBI:34887
nickel dichloride
|
|
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
126.2 MILLIMOLAR |
CHEBI:17234
glucose
|
|
| 4 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.6802 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
|
17.11 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 6 |
Cupric sulfate
Cu.O4S 159.609 g/mol
|
0.005006 MILLIMOLAR |
CHEBI:23414
copper(II) sulfate
|
|
| 7 |
Zinc sulfate
O4S.Zn 161.453 g/mol
|
0.02487 MILLIMOLAR |
CHEBI:35176
zinc sulfate
|
|
| 8 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
4.057 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 9 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
11.02 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
55.24 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:45.437688Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 312, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:46.896863Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007211 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:05.080786+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CDM for Batch Cultivation" to "cdm_for_batch_cultivation" for consistent matching |
| 2026-06-05T07:40:52.746816+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |