chamberlain_cdm

CultureMech:007212 bacterial DEFINED LIQUID
Original name: Chamberlain CDM

Overview

External ontology
MEDIADB:313 (source link unavailable) — Chamberlain CDM
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007212["chamberlain_cdm"]
    ing0_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007212 --> ing0_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing0_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing1_L_Arginine["L-Arginine"]
    CultureMech_007212 --> ing1_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing1_L_Arginine -.-> CHEBI_16467
    ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007212 --> ing2_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007212 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Glucose["Glucose"]
    CultureMech_007212 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007212 --> ing5_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing5_Ferrous_sulfate -.-> CHEBI_75832
    ing6_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007212 --> ing6_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing6_Magnesium_sulfate -.-> CHEBI_32599
    ing7_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007212 --> ing7_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing7_Calcium_pantothenate -.-> CHEBI_31345
    ing8_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007212 --> ing8_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing8_Thiamine_HCl -.-> CHEBI_49105
    ing9_Threonine["Threonine"]
    CultureMech_007212 --> ing9_Threonine
    CHEBI_26986["CHEBI:26986"]
    ing9_Threonine -.-> CHEBI_26986
    ing10_DL_methionine["DL-methionine"]
    CultureMech_007212 --> ing10_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing10_DL_methionine -.-> CHEBI_16811
    ing11_Isoleucine["Isoleucine"]
    CultureMech_007212 --> ing11_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing11_Isoleucine -.-> CHEBI_17191
    ing12_Spermine_phosphate["Spermine phosphate"]
    CultureMech_007212 --> ing12_Spermine_phosphate
    ing13_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_007212 --> ing13_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing13_Cysteine_HCl -.-> CHEBI_91247
    ing14_L_Leucine["L-Leucine"]
    CultureMech_007212 --> ing14_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing14_L_Leucine -.-> CHEBI_15603
    ing15_Valine["Valine"]
    CultureMech_007212 --> ing15_Valine
    CHEBI_16414["CHEBI:16414"]
    ing15_Valine -.-> CHEBI_16414
    ing16_L_Histidine["L-Histidine"]
    CultureMech_007212 --> ing16_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing16_L_Histidine -.-> CHEBI_15971
    ing17_L_Proline["L-Proline"]
    CultureMech_007212 --> ing17_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing17_L_Proline -.-> CHEBI_17203
    ing18_L_Aspartate["L-Aspartate"]
    CultureMech_007212 --> ing18_L_Aspartate
    CHEBI_29991["CHEBI:29991"]
    ing18_L_Aspartate -.-> CHEBI_29991
    ing19_L_Lysine["L-Lysine"]
    CultureMech_007212 --> ing19_L_Lysine
    CHEBI_18019["CHEBI:18019"]
    ing19_L_Lysine -.-> CHEBI_18019
    ing20_DL_Serine["DL-Serine"]
    CultureMech_007212 --> ing20_DL_Serine
    ing21_L_Tyrosine["L-Tyrosine"]
    CultureMech_007212 --> ing21_L_Tyrosine
    CHEBI_17895["CHEBI:17895"]
    ing21_L_Tyrosine -.-> CHEBI_17895

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
7.348 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
2 L-Arginine
C6H14N4O2 174.204 g/mol
2.296 MILLIMOLAR CHEBI:16467
L-arginine
 Cross-references: KEGG:arg-L
3 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
5.741 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
4 Sodium chloride
Cl.Na 58.443 g/mol
171.12 MILLIMOLAR CHEBI:26710
sodium chloride
5 Glucose
C6H12O6 180.156 g/mol
22.2 MILLIMOLAR CHEBI:17234
glucose
6 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.007194 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
7 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.5477 MILLIMOLAR CHEBI:32599
magnesium sulfate
8 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.004197 MILLIMOLAR CHEBI:31345
Calcium pantothenate
9 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.01186 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
10 Threonine
C4H9NO3 119.119 g/mol
16.79 MILLIMOLAR CHEBI:26986
threonine
11 DL-methionine
C5H11NO2S 149.215 g/mol
2.681 MILLIMOLAR CHEBI:16811
methionine
12 Isoleucine
C6H13NO2 131.175 g/mol
3.049 MILLIMOLAR CHEBI:17191
L-isoleucine
13 Spermine phosphate 0.1004 MILLIMOLAR
14 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
1.269 MILLIMOLAR CHEBI:91247
L-cysteine hydrochloride
15 L-Leucine
C6H13NO2 131.175 g/mol
3.049 MILLIMOLAR CHEBI:15603
L-leucine
 Cross-references: KEGG:leu-L
16 Valine
C5H11NO2 117.148 g/mol
3.414 MILLIMOLAR CHEBI:16414
L-valine
17 L-Histidine
C6H9N3O2 155.157 g/mol
1.289 MILLIMOLAR CHEBI:15971
L-histidine
 Cross-references: KEGG:his-L
18 L-Proline
C5H9NO2 115.132 g/mol
17.37 MILLIMOLAR CHEBI:17203
L-proline
 Cross-references: KEGG:pro-L
19 L-Aspartate
C4H6NO4 132.095 g/mol
3.005 MILLIMOLAR CHEBI:29991
L-aspartate(1-)
 Cross-references: KEGG:asp-L
20 L-Lysine
C6H14N2O2 146.19 g/mol
2.736 MILLIMOLAR CHEBI:18019
L-lysine
 Cross-references: KEGG:lys-L
21 DL-Serine 3.806 MILLIMOLAR
22 L-Tyrosine
C9H11NO3 181.191 g/mol
2.208 MILLIMOLAR CHEBI:17895
L-tyrosine
 Cross-references: KEGG:tyr-L

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.445936Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 313, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:46.905141Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007212
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.089130+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Chamberlain CDM" to "chamberlain_cdm" for consistent matching
2026-06-05T07:40:53.049650+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:37.022579+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 2 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).