hypertonic_medium (CultureMech:007215)

Overview

External ontology

MEDIADB:316 (source link unavailable) — Hypertonic medium

Applications

Cultivation of genome-sequenced organisms
Metabolic modeling
Systems biology research

Notes

Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007215["hypertonic_medium"]
    ing0_L_Leucine["L-Leucine"]
    CultureMech_007215 --> ing0_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing0_L_Leucine -.-> CHEBI_15603
    ing1_Sucrose["Sucrose"]
    CultureMech_007215 --> ing1_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing1_Sucrose -.-> CHEBI_17992
    ing2_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007215 --> ing2_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing2_Ammonium_chloride -.-> CHEBI_31206
    ing3_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007215 --> ing3_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing3_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing4_Sodium_sulfate["Sodium sulfate"]
    CultureMech_007215 --> ing4_Sodium_sulfate
    CHEBI_32149["CHEBI:32149"]
    ing4_Sodium_sulfate -.-> CHEBI_32149
    ing5_Glucose["Glucose"]
    CultureMech_007215 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007215 --> ing6_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing6_Magnesium_chloride -.-> CHEBI_86345
    ing7_Sodium_chloride["Sodium chloride"]
    CultureMech_007215 --> ing7_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing7_Sodium_chloride -.-> CHEBI_26710
    ing8_Potassium_chloride["Potassium chloride"]
    CultureMech_007215 --> ing8_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing8_Potassium_chloride -.-> CHEBI_32588
    ing9_Tromethamine["Tromethamine"]
    CultureMech_007215 --> ing9_Tromethamine
    CHEBI_9754["CHEBI:9754"]
    ing9_Tromethamine -.-> CHEBI_9754

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	L-Leucine C6H13NO2 131.175 g/mol	0.3812 MILLIMOLAR	CHEBI:15603 L-leucine	Cross-references: KEGG:leu-L
2	Sucrose C12H22O11 342.297 g/mol	200.0 MILLIMOLAR	CHEBI:17992 sucrose	Cross-references: KEGG:sucr
3	Ammonium chloride Cl.H4N 53.492 g/mol	18.7 MILLIMOLAR	CHEBI:31206 ammonium chloride
4	Potassium dihydrogen phosphate H2O4P.K 136.084 g/mol	1.029 MILLIMOLAR	CHEBI:63036 potassium dihydrogen phosphate
5	Sodium sulfate 2Na.O4S 142.043 g/mol	0.9311 MILLIMOLAR	CHEBI:32149 sodium sulfate
6	Glucose C6H12O6 180.156 g/mol	11.1 MILLIMOLAR	CHEBI:17234 glucose
7	Magnesium chloride 2Cl.6H2O.Mg 203.301 g/mol	22.99 MILLIMOLAR	CHEBI:86345 magnesium dichloride hexahydrate
8	Sodium chloride Cl.Na 58.443 g/mol	0.9925 MILLIMOLAR	CHEBI:26710 sodium chloride
9	Potassium chloride Cl.K 74.551 g/mol	0.4695 MILLIMOLAR	CHEBI:32588 potassium chloride
10	Tromethamine C4H11NO3 121.136 g/mol	99.06 MILLIMOLAR	CHEBI:9754 tris

Preparation

Dissolve all ingredients in distilled water to specified concentrations
Adjust pH if specified in original formulation
Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T07:21:45.478344Z	mediadb-import	Imported from MediaDB	Source: MediaDB (Institute for Systems Biology), Medium ID: 316, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:14.837053Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:46.937654Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007215
2026-03-17T06:29:57.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.123355+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Hypertonic medium" to "hypertonic_medium" for consistent matching
2026-06-05T07:41:09.189555+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.