labarre_modified_bg11

CultureMech:007221 bacterial DEFINED LIQUID
Original name: Labarre (Modified BG11)

Overview

External ontology
MEDIADB:321 (source link unavailable) — Labarre (Modified BG11)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007221["labarre_modified_bg11"]
    ing0_Sodium_nitrate["Sodium nitrate"]
    CultureMech_007221 --> ing0_Sodium_nitrate
    CHEBI_63005["CHEBI:63005"]
    ing0_Sodium_nitrate -.-> CHEBI_63005
    ing1_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007221 --> ing1_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing1_Sodium_molybdate -.-> CHEBI_75215
    ing2_HEPES["HEPES"]
    CultureMech_007221 --> ing2_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing2_HEPES -.-> CHEBI_46756
    ing3_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007221 --> ing3_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing3_Cupric_sulfate -.-> CHEBI_23414
    ing4_Manganese_chloride["Manganese chloride"]
    CultureMech_007221 --> ing4_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing4_Manganese_chloride -.-> CHEBI_63041
    ing5_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007221 --> ing5_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing5_Zinc_sulfate -.-> CHEBI_35176
    ing6_Cobalt_nitrate["Cobalt nitrate"]
    CultureMech_007221 --> ing6_Cobalt_nitrate
    CHEBI_86209["CHEBI:86209"]
    ing6_Cobalt_nitrate -.-> CHEBI_86209
    ing7_Boric_acid["Boric acid"]
    CultureMech_007221 --> ing7_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing7_Boric_acid -.-> CHEBI_33118
    ing8_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007221 --> ing8_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing8_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing9_Citrate["Citrate"]
    CultureMech_007221 --> ing9_Citrate
    CHEBI_16947["CHEBI:16947"]
    ing9_Citrate -.-> CHEBI_16947
    ing10_EDTA["EDTA"]
    CultureMech_007221 --> ing10_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing10_EDTA -.-> CHEBI_4735
    ing11_sodium_silicate["sodium silicate"]
    CultureMech_007221 --> ing11_sodium_silicate
    CHEBI_60720["CHEBI:60720"]
    ing11_sodium_silicate -.-> CHEBI_60720
    ing12_Sodium_carbonate["Sodium carbonate"]
    CultureMech_007221 --> ing12_Sodium_carbonate
    CHEBI_29377["CHEBI:29377"]
    ing12_Sodium_carbonate -.-> CHEBI_29377
    ing13_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007221 --> ing13_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing13_Magnesium_sulfate -.-> CHEBI_32599
    ing14_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007221 --> ing14_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing14_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing15_Fe_III_dicitrate["Fe(III)dicitrate"]
    CultureMech_007221 --> ing15_Fe_III_dicitrate

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Sodium nitrate
NO3.Na 84.994 g/mol
10.03 MILLIMOLAR CHEBI:63005
sodium nitrate
2 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.001616 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
3 HEPES 6.0 MILLIMOLAR CHEBI:46756
HEPES
4 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0003164 MILLIMOLAR CHEBI:23414
copper(II) sulfate
5 Manganese chloride
Cl2Mn 125.844 g/mol
0.009145 MILLIMOLAR CHEBI:63041
manganese(II) chloride
6 Zinc sulfate
O4S.Zn 161.453 g/mol
0.0007721 MILLIMOLAR CHEBI:35176
zinc sulfate
7 Cobalt nitrate
Co.2NO3 182.941 g/mol
0.0001697 MILLIMOLAR CHEBI:86209
cobalt dinitrate
8 Boric acid
H3BO3 61.833 g/mol
0.04626 MILLIMOLAR CHEBI:33118
boric acid
9 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.2433 MILLIMOLAR CHEBI:3312
calcium dichloride
10 Citrate
C6H5O7 189.099 g/mol
0.03123 MILLIMOLAR CHEBI:16947
citrate(3-)
 Cross-references: KEGG:cit
11 EDTA
C10H16N2O8 292.244 g/mol
0.003422 MILLIMOLAR CHEBI:4735
ethylenediaminetetraacetic acid
12 sodium silicate
2Na.O3Si 122.063 g/mol
0.2041 MILLIMOLAR CHEBI:60720
sodium silicate
13 Sodium carbonate
CO3.2Na 105.988 g/mol
1.887 MILLIMOLAR CHEBI:29377
sodium carbonate
14 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.3043 MILLIMOLAR CHEBI:32599
magnesium sulfate
15 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
0.2239 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
16 Fe(III)dicitrate 0.0245 MILLIMOLAR Cross-references: KEGG:fe3dcit

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.522450Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 321, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:15.540864Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:46.971689Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007221
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.159302+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Labarre (Modified BG11" to "labarre_modified_bg11" for consistent matching
2026-06-05T07:41:10.627212+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:06:24.112486+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:321's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.
2026-08-31T06:17:35.464974+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.