chemically_defined_fermentation_medium_20g_glucose_20g_glycerol_12_5g_msg

CultureMech:007230 bacterial DEFINED LIQUID
Original name: Chemically Defined Fermentation Medium (20g glucose + 20g glycerol +12.5g MSG)

Overview

External ontology
MEDIADB:330 (source link unavailable) — Chemically Defined Fermentation Medium (20g glucose + 20g glycerol +12.5g MSG)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007230["chemically_defined_fermentation_medium_20g_glucose_20g_glycerol_12_..."]
    ing0_Boric_acid["Boric acid"]
    CultureMech_007230 --> ing0_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing0_Boric_acid -.-> CHEBI_33118
    ing1_Glycerol["Glycerol"]
    CultureMech_007230 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007230 --> ing2_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing3_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007230 --> ing3_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing3_Manganese_sulfate -.-> CHEBI_86364
    ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007230 --> ing4_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing5_Glucose["Glucose"]
    CultureMech_007230 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007230 --> ing6_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing6_Magnesium_sulfate -.-> CHEBI_32599
    ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007230 --> ing7_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing8_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007230 --> ing8_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing8_Ferrous_sulfate -.-> CHEBI_75832
    ing9_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007230 --> ing9_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing9_Zinc_sulfate -.-> CHEBI_35176
    ing10_Cupric_chloride["Cupric chloride"]
    CultureMech_007230 --> ing10_Cupric_chloride
    CHEBI_49553["CHEBI:49553"]
    ing10_Cupric_chloride -.-> CHEBI_49553
    ing11_Ammonium_heptamolybdate_tetrahydrate["Ammonium heptamolybdate tetrahydrate"]
    CultureMech_007230 --> ing11_Ammonium_heptamolybdate_tetrahydrate
    ing12_Sodium_Glutamate["Sodium Glutamate"]
    CultureMech_007230 --> ing12_Sodium_Glutamate
    CHEBI_64220["CHEBI:64220"]
    ing12_Sodium_Glutamate -.-> CHEBI_64220

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Boric acid
H3BO3 61.833 g/mol
0.1779 MILLIMOLAR CHEBI:33118
boric acid
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
2 Glycerol
C3H8O3 92.094 g/mol
217.18 MILLIMOLAR CHEBI:17754
glycerol
 Cross-references: KEGG:glyc This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
3 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.136 MILLIMOLAR CHEBI:3312
calcium dichloride
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
4 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.4483 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
5 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
28.71 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
6 Glucose
C6H12O6 180.156 g/mol
111.01 MILLIMOLAR CHEBI:17234
glucose
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
7 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.4057 MILLIMOLAR CHEBI:32599
magnesium sulfate
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
8 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
36.74 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
9 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.7194 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
10 Zinc sulfate
O4S.Zn 161.453 g/mol
0.6955 MILLIMOLAR CHEBI:35176
zinc sulfate
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
11 Cupric chloride
Cl2Cu 134.452 g/mol
0.02933 MILLIMOLAR CHEBI:49553
copper(II) chloride
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.
12 Ammonium heptamolybdate tetrahydrate 0.004045 MILLIMOLAR This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance. CHEBI:91249 identifies orthomolybdate, not the source heptamolybdate tetrahydrate. The conflicting mapping is withdrawn; no replacement is assigned and the MIM snapshot is unchanged.
13 Sodium Glutamate
C5H8NO4.Na 169.112 g/mol
66.8 MILLIMOLAR CHEBI:64220
monosodium glutamate
 This amount is the MediaDB assertion; its page and dated export have no linked primary article. The Hajjaj paired-carbon formulation is a source lead, not established provenance.

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.607336Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 330, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:27.503693Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:47.042645Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007230
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.232554+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Chemically Defined Fermentation Medium (20g glucose + 20g glycerol +12.5g MSG" to "chemically_defined_fermentation_medium_20g_glucose_20g_glycerol_12_5g_msg" for consistent matching
2026-06-05T07:40:53.092215+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:17:23.397066+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.
2026-10-10T05:02:53.552216+00:00 codex CURATED_HAJJAJ_SOURCED_INGREDIENT_AMOUNTS_AND_FORMS The 180 retained mineral molarities in article-linked records remain upstream database assertions; water-input basis is not assumed to be final solution volume. The two unlinked sibling records retain all 26 database molarities. Their primary-paper association and remaining chemical forms are unresolved, not borrowed from the linked family. Copper and sodium-glutamate hydrate identifiers remain unassigned after withdrawal of unverified non-hydrate grounding. Isoleucine stereochemistry is unspecified, not inferred. Heptamolybdate has no replacement identifier; the conflicting packaged MIM synonym is not used and the immutable index is unchanged. The source's Table 2 glutamic-acid equivalents are not the weighed sodium-glutamate hydrate; record 318 retains the batch-text/Figure 3 glucose discrepancy in evidence. Paper and ontology verification is manual; no raw primary-article capture or successful automated DOI full-text verification is asserted. Existing preparation, classification, application and organism/role claims are preserved and not certified by this ingredient curation. No IDs, category paths, growth claims or taxa-media associations are added.