chemically_defined_fermentation_medium_45g_glucose_4g_ammonium_nitrate

CultureMech:007233 bacterial DEFINED LIQUID
Original name: Chemically Defined Fermentation Medium (45g glucose + 4g ammonium nitrate)

Overview

External ontology
MEDIADB:333 (source link unavailable) — Chemically Defined Fermentation Medium (45g glucose + 4g ammonium nitrate)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007233["chemically_defined_fermentation_medium_45g_glucose_4g_ammonium_nitrate"]
    ing0_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007233 --> ing0_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing0_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing1_Glucose["Glucose"]
    CultureMech_007233 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Boric_acid["Boric acid"]
    CultureMech_007233 --> ing2_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing2_Boric_acid -.-> CHEBI_33118
    ing3_iron_2___sulfate_heptahydrate["iron(2+) sulfate heptahydrate"]
    CultureMech_007233 --> ing3_iron_2___sulfate_heptahydrate
    CHEBI_75836["CHEBI:75836"]
    ing3_iron_2___sulfate_heptahydrate -.-> CHEBI_75836
    ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007233 --> ing4_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing5_calcium_chloride_dihydrate["calcium chloride dihydrate"]
    CultureMech_007233 --> ing5_calcium_chloride_dihydrate
    CHEBI_86158["CHEBI:86158"]
    ing5_calcium_chloride_dihydrate -.-> CHEBI_86158
    ing6_manganese_II__sulfate_tetrahydrate["manganese(II) sulfate tetrahydrate"]
    CultureMech_007233 --> ing6_manganese_II__sulfate_tetrahydrate
    CHEBI_86358["CHEBI:86358"]
    ing6_manganese_II__sulfate_tetrahydrate -.-> CHEBI_86358
    ing7_magnesium_sulfate_heptahydrate["magnesium sulfate heptahydrate"]
    CultureMech_007233 --> ing7_magnesium_sulfate_heptahydrate
    CHEBI_31795["CHEBI:31795"]
    ing7_magnesium_sulfate_heptahydrate -.-> CHEBI_31795
    ing8_zinc_sulfate_heptahydrate["zinc sulfate heptahydrate"]
    CultureMech_007233 --> ing8_zinc_sulfate_heptahydrate
    CHEBI_32312["CHEBI:32312"]
    ing8_zinc_sulfate_heptahydrate -.-> CHEBI_32312
    ing9_copper_II__chloride_dihydrate["copper(II) chloride dihydrate"]
    CultureMech_007233 --> ing9_copper_II__chloride_dihydrate
    ing10_Ammonium_heptamolybdate_tetrahydrate["Ammonium heptamolybdate tetrahydrate"]
    CultureMech_007233 --> ing10_Ammonium_heptamolybdate_tetrahydrate
    ing11_Ammonium_nitrate["Ammonium nitrate"]
    CultureMech_007233 --> ing11_Ammonium_nitrate
    CHEBI_63038["CHEBI:63038"]
    ing11_Ammonium_nitrate -.-> CHEBI_63038

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
28.71 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 Primary recipe input: 5 g K2HPO4 per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
2 Glucose
C6H12O6 180.156 g/mol
45 G_PER_L CHEBI:17234
glucose
 The source's initial 45 g/L input replaces MediaDB 249.8 mM; this is source-unit restoration, not a claim that the database conversion was numerically wrong.
3 Boric acid
H3BO3 61.833 g/mol
0.1779 MILLIMOLAR CHEBI:33118
boric acid
 Primary recipe input: 11 mg H3BO3 per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
4 iron(2+) sulfate heptahydrate
Fe.7H2O.O4S 278.013 g/mol
0.7194 MILLIMOLAR CHEBI:75836
iron(2+) sulfate heptahydrate
 Primary recipe input: 0.2 g FeSO4.7H2O per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
5 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
36.74 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
 Primary recipe input: 5 g KH2PO4 per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
6 calcium chloride dihydrate
Ca.2Cl.2H2O 147.014 g/mol
0.136 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 Primary recipe input: 20 mg CaCl2.2H2O per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
7 manganese(II) sulfate tetrahydrate
4H2O.Mn.O4S 223.061 g/mol
0.4483 MILLIMOLAR CHEBI:86358
manganese(II) sulfate tetrahydrate
 Primary recipe input: 0.1 g MnSO4.4H2O per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
8 magnesium sulfate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
0.4057 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 Primary recipe input: 0.1 g MgSO4.7H2O per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
9 zinc sulfate heptahydrate
7H2O.O4S.Zn 287.558 g/mol
0.6955 MILLIMOLAR CHEBI:32312
zinc sulfate heptahydrate
 Primary recipe input: 0.2 g ZnSO4.7H2O per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied.
10 copper(II) chloride dihydrate 0.02933 MILLIMOLAR Primary recipe input: 5 mg CuCl2.2H2O per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied. No exact supplied-hydrate identifier is assigned: the packaged candidate's public ChEBI page was unavailable. The prior non-hydrate grounding is withdrawn, not a new identifier inferred.
11 Ammonium heptamolybdate tetrahydrate 0.004045 MILLIMOLAR Primary recipe input: 5 mg (NH4)6Mo7O24.4H2O per 1 L distilled water. Final solution volume is not explicit. The stored molarity is retained as a database assertion, not independently verified final-medium concentration; no conversion was applied. CHEBI:91249 identifies orthomolybdate, not the source heptamolybdate tetrahydrate. The conflicting mapping is withdrawn; no replacement is assigned and the MIM snapshot is unchanged.
12 Ammonium nitrate
H4N.NO3 80.043 g/mol
4 G_PER_L CHEBI:63038
ammonium nitrate
 The source's initial 4 g/L input replaces MediaDB 49.98 mM; this is source-unit restoration, not a claim that the database conversion was numerically wrong.

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:45.639660Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 333, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:47.061436Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007233
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.252548+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Chemically Defined Fermentation Medium (45g glucose + 4g ammonium nitrate" to "chemically_defined_fermentation_medium_45g_glucose_4g_ammonium_nitrate" for consistent matching
2026-06-05T07:40:53.189126+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:17:23.499703+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.
2026-10-10T05:02:53.552216+00:00 codex CURATED_HAJJAJ_SOURCED_INGREDIENT_AMOUNTS_AND_FORMS The 180 retained mineral molarities in article-linked records remain upstream database assertions; water-input basis is not assumed to be final solution volume. The two unlinked sibling records retain all 26 database molarities. Their primary-paper association and remaining chemical forms are unresolved, not borrowed from the linked family. Copper and sodium-glutamate hydrate identifiers remain unassigned after withdrawal of unverified non-hydrate grounding. Isoleucine stereochemistry is unspecified, not inferred. Heptamolybdate has no replacement identifier; the conflicting packaged MIM synonym is not used and the immutable index is unchanged. The source's Table 2 glutamic-acid equivalents are not the weighed sodium-glutamate hydrate; record 318 retains the batch-text/Figure 3 glucose discrepancy in evidence. Paper and ontology verification is manual; no raw primary-article capture or successful automated DOI full-text verification is asserted. Existing preparation, classification, application and organism/role claims are preserved and not certified by this ingredient curation. No IDs, category paths, growth claims or taxa-media associations are added.