modified_labarre_medium_56_mm_glucose

CultureMech:007290 bacterial DEFINED LIQUID
Original name: Modified Labarre Medium (56 mM Glucose)

Overview

External ontology
MEDIADB:386 (source link unavailable) — Modified Labarre Medium (56 mM Glucose)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007290["modified_labarre_medium_56_mm_glucose"]
    ing0_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007290 --> ing0_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing0_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing1_Citrate["Citrate"]
    CultureMech_007290 --> ing1_Citrate
    CHEBI_16947["CHEBI:16947"]
    ing1_Citrate -.-> CHEBI_16947
    ing2_Fe_III_dicitrate["Fe(III)dicitrate"]
    CultureMech_007290 --> ing2_Fe_III_dicitrate
    ing3_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007290 --> ing3_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing3_Magnesium_sulfate -.-> CHEBI_32599
    ing4_Boric_acid["Boric acid"]
    CultureMech_007290 --> ing4_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing4_Boric_acid -.-> CHEBI_33118
    ing5_EDTA["EDTA"]
    CultureMech_007290 --> ing5_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing5_EDTA -.-> CHEBI_4735
    ing6_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007290 --> ing6_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing6_Sodium_molybdate -.-> CHEBI_75215
    ing7_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007290 --> ing7_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing7_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing8_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007290 --> ing8_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing8_Zinc_sulfate -.-> CHEBI_35176
    ing9_Cobalt_nitrate["Cobalt nitrate"]
    CultureMech_007290 --> ing9_Cobalt_nitrate
    CHEBI_86209["CHEBI:86209"]
    ing9_Cobalt_nitrate -.-> CHEBI_86209
    ing10_HEPES["HEPES"]
    CultureMech_007290 --> ing10_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing10_HEPES -.-> CHEBI_46756
    ing11_Sodium_carbonate["Sodium carbonate"]
    CultureMech_007290 --> ing11_Sodium_carbonate
    CHEBI_29377["CHEBI:29377"]
    ing11_Sodium_carbonate -.-> CHEBI_29377
    ing12_Glucose["Glucose"]
    CultureMech_007290 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_Manganese_chloride["Manganese chloride"]
    CultureMech_007290 --> ing13_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing13_Manganese_chloride -.-> CHEBI_63041
    ing14_Sodium_nitrate["Sodium nitrate"]
    CultureMech_007290 --> ing14_Sodium_nitrate
    CHEBI_63005["CHEBI:63005"]
    ing14_Sodium_nitrate -.-> CHEBI_63005
    ing15_sodium_silicate["sodium silicate"]
    CultureMech_007290 --> ing15_sodium_silicate
    CHEBI_60720["CHEBI:60720"]
    ing15_sodium_silicate -.-> CHEBI_60720
    ing16_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007290 --> ing16_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing16_Cupric_sulfate -.-> CHEBI_23414

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.2433 MILLIMOLAR CHEBI:3312
calcium dichloride
2 Citrate
C6H5O7 189.099 g/mol
0.03123 MILLIMOLAR CHEBI:16947
citrate(3-)
 Cross-references: KEGG:cit
3 Fe(III)dicitrate 0.0245 MILLIMOLAR Cross-references: KEGG:fe3dcit
4 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.3043 MILLIMOLAR CHEBI:32599
magnesium sulfate
5 Boric acid
H3BO3 61.833 g/mol
0.04626 MILLIMOLAR CHEBI:33118
boric acid
6 EDTA
C10H16N2O8 292.244 g/mol
0.003422 MILLIMOLAR CHEBI:4735
ethylenediaminetetraacetic acid
7 Sodium molybdate
MoO4.2Na 205.916 g/mol
0.001616 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
8 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
0.2239 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
9 Zinc sulfate
O4S.Zn 161.453 g/mol
0.0007721 MILLIMOLAR CHEBI:35176
zinc sulfate
10 Cobalt nitrate
Co.2NO3 182.941 g/mol
0.0001697 MILLIMOLAR CHEBI:86209
cobalt dinitrate
11 HEPES 6.0 MILLIMOLAR CHEBI:46756
HEPES
12 Sodium carbonate
CO3.2Na 105.988 g/mol
1.887 MILLIMOLAR CHEBI:29377
sodium carbonate
13 Glucose
C6H12O6 180.156 g/mol
56.0 MILLIMOLAR CHEBI:17234
glucose
14 Manganese chloride
Cl2Mn 125.844 g/mol
0.009145 MILLIMOLAR CHEBI:63041
manganese(II) chloride
15 Sodium nitrate
NO3.Na 84.994 g/mol
10.03 MILLIMOLAR CHEBI:63005
sodium nitrate
16 sodium silicate
2Na.O3Si 122.063 g/mol
0.2041 MILLIMOLAR CHEBI:60720
sodium silicate
17 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0003164 MILLIMOLAR CHEBI:23414
copper(II) sulfate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.116099Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 386, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:09.815584Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:47.447514Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007290
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.674190+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Modified Labarre Medium (56 mM Glucose" to "modified_labarre_medium_56_mm_glucose" for consistent matching
2026-06-05T07:41:36.776642+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:07:04.118592+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:386's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.
2026-08-31T06:18:16.996489+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.