nitrogen_limited_mr_medium

CultureMech:007295 bacterial DEFINED LIQUID
Original name: Nitrogen-limited MR medium

Overview

External ontology
MEDIADB:391 (source link unavailable) — Nitrogen-limited MR medium
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007295["nitrogen_limited_mr_medium"]
    ing0_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007295 --> ing0_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing0_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing1_D_Fructose["D-Fructose"]
    CultureMech_007295 --> ing1_D_Fructose
    CHEBI_15824["CHEBI:15824"]
    ing1_D_Fructose -.-> CHEBI_15824
    ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007295 --> ing2_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing3_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007295 --> ing3_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing3_Zinc_sulfate -.-> CHEBI_35176
    ing4_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007295 --> ing4_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_sulfate -.-> CHEBI_32599
    ing5_Citrate["Citrate"]
    CultureMech_007295 --> ing5_Citrate
    CHEBI_16947["CHEBI:16947"]
    ing5_Citrate -.-> CHEBI_16947
    ing6_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007295 --> ing6_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing6_Ferrous_sulfate -.-> CHEBI_75832
    ing7_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007295 --> ing7_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing7_Manganese_sulfate -.-> CHEBI_86364
    ing8_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007295 --> ing8_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing8_Cupric_sulfate -.-> CHEBI_23414
    ing9_Sodium_borate["Sodium borate"]
    CultureMech_007295 --> ing9_Sodium_borate
    ing10_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
    CultureMech_007295 --> ing10_Molybdic_acid_ammonium_salt_tetrahydrate
    CHEBI_91249["CHEBI:91249"]
    ing10_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
    ing11_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007295 --> ing11_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing11_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing12_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007295 --> ing12_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing12_Ammonium_chloride -.-> CHEBI_31206

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
49.01 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
2 D-Fructose
C6H12O6 180.156 g/mol
111.0 MILLIMOLAR CHEBI:15824
D-fructose
 Cross-references: KEGG:fru
3 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.09011 MILLIMOLAR CHEBI:3312
calcium dichloride
4 Zinc sulfate
O4S.Zn 161.453 g/mol
0.03825 MILLIMOLAR CHEBI:35176
zinc sulfate
5 Magnesium sulfate
Mg.O4S 120.368 g/mol
4.587 MILLIMOLAR CHEBI:32599
magnesium sulfate
6 Citrate
C6H5O7 189.099 g/mol
4.164 MILLIMOLAR CHEBI:16947
citrate(3-)
 Cross-references: KEGG:cit
7 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.1798 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
8 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.01121 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
9 Cupric sulfate
Cu.O4S 159.609 g/mol
0.02002 MILLIMOLAR CHEBI:23414
copper(II) sulfate
10 Sodium borate 0.0002622 MILLIMOLAR
11 Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
0.0004045 MILLIMOLAR CHEBI:91249
ammonium molybdate
12 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
33.34 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
13 Ammonium chloride
Cl.H4N 53.492 g/mol
33.65 MILLIMOLAR CHEBI:31206
ammonium chloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.165622Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 391, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:47.502207Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007295
2026-03-17T06:30:35.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.737896+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nitrogen-limited MR medium" to "nitrogen_limited_mr_medium" for consistent matching
2026-06-05T07:41:39.803873+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.