minimal_chemically_defined_medium_his_pro_letort_2001

CultureMech:007297 bacterial DEFINED LIQUID
Original name: Minimal chemically-defined medium + His +Pro (letort 2001)

Overview

External ontology
MEDIADB:393 (source link unavailable) — Minimal chemically-defined medium + His +Pro (letort 2001)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007297["minimal_chemically_defined_medium_his_pro_letort_2001"]
    ing0_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007297 --> ing0_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing0_Thiamine_HCl -.-> CHEBI_49105
    ing1_Riboflavin["Riboflavin"]
    CultureMech_007297 --> ing1_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing1_Riboflavin -.-> CHEBI_17015
    ing2_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007297 --> ing2_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing2_Magnesium_chloride -.-> CHEBI_86345
    ing3_DL_methionine["DL-methionine"]
    CultureMech_007297 --> ing3_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing3_DL_methionine -.-> CHEBI_16811
    ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007297 --> ing4_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing5_Cysteine["Cysteine"]
    CultureMech_007297 --> ing5_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing5_Cysteine -.-> CHEBI_15356
    ing6_Ammonium_citrate["Ammonium citrate"]
    CultureMech_007297 --> ing6_Ammonium_citrate
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_007297 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Proline["Proline"]
    CultureMech_007297 --> ing8_Proline
    CHEBI_26271["CHEBI:26271"]
    ing8_Proline -.-> CHEBI_26271
    ing9_Valine["Valine"]
    CultureMech_007297 --> ing9_Valine
    CHEBI_16414["CHEBI:16414"]
    ing9_Valine -.-> CHEBI_16414
    ing10_Histidine["Histidine"]
    CultureMech_007297 --> ing10_Histidine
    CHEBI_27570["CHEBI:27570"]
    ing10_Histidine -.-> CHEBI_27570
    ing11_Nicotinate["Nicotinate"]
    CultureMech_007297 --> ing11_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing11_Nicotinate -.-> CHEBI_15940
    ing12_Pyridoxamine_HCl["Pyridoxamine HCl"]
    CultureMech_007297 --> ing12_Pyridoxamine_HCl
    CHEBI_131531["CHEBI:131531"]
    ing12_Pyridoxamine_HCl -.-> CHEBI_131531
    ing13_Ascorbate["Ascorbate"]
    CultureMech_007297 --> ing13_Ascorbate
    CHEBI_22651["CHEBI:22651"]
    ing13_Ascorbate -.-> CHEBI_22651
    ing14_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007297 --> ing14_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing14_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing15_Isoleucine["Isoleucine"]
    CultureMech_007297 --> ing15_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing15_Isoleucine -.-> CHEBI_17191
    ing16_Leucine["Leucine"]
    CultureMech_007297 --> ing16_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing16_Leucine -.-> CHEBI_15603
    ing17_L_Glutamate["L-Glutamate"]
    CultureMech_007297 --> ing17_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing17_L_Glutamate -.-> CHEBI_16015
    ing18_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007297 --> ing18_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_pantothenate -.-> CHEBI_31345
    ing19_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007297 --> ing19_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing19_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing20_Lactose["Lactose"]
    CultureMech_007297 --> ing20_Lactose
    CHEBI_17716["CHEBI:17716"]
    ing20_Lactose -.-> CHEBI_17716

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.002965 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
2 Riboflavin
C17H20N4O6 376.369 g/mol
0.002657 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
3 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.9838 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
4 DL-methionine
C5H11NO2S 149.215 g/mol
0.8713 MILLIMOLAR CHEBI:16811
methionine
5 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
22.04 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
6 Cysteine
C3H7NO2S 121.161 g/mol
2.063 MILLIMOLAR CHEBI:15356
cysteine
7 Ammonium citrate 28.69 MILLIMOLAR
8 Sodium acetate
C2H3O2.Na 82.034 g/mol
12.19 MILLIMOLAR CHEBI:32954
sodium acetate
9 Proline
C5H9NO2 115.132 g/mol
5.906 MILLIMOLAR CHEBI:26271
proline
10 Valine
C5H11NO2 117.148 g/mol
2.817 MILLIMOLAR CHEBI:16414
L-valine
11 Histidine
C6H9N3O2 155.157 g/mol
0.9667 MILLIMOLAR CHEBI:27570
histidine
12 Nicotinate
C6H5NO2 123.111 g/mol
0.008123 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
13 Pyridoxamine HCl
C8H12N2O2.HCl 204.657 g/mol
0.02443 MILLIMOLAR CHEBI:131531
pyridoxamine hydrochloride
14 Ascorbate 2.839 MILLIMOLAR CHEBI:22651
ascorbate
 Cross-references: KEGG:ascb-L
15 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.3401 MILLIMOLAR CHEBI:3312
calcium dichloride
16 Isoleucine
C6H13NO2 131.175 g/mol
1.677 MILLIMOLAR CHEBI:17191
L-isoleucine
17 Leucine
C6H13NO2 131.175 g/mol
3.659 MILLIMOLAR CHEBI:15603
L-leucine
18 L-Glutamate
C5H9NO4 147.13 g/mol
2.719 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
19 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.002099 MILLIMOLAR CHEBI:31345
Calcium pantothenate
20 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
13.41 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
21 Lactose
C12H22O11 342.297 g/mol
29.21 MILLIMOLAR CHEBI:17716
lactose
 Cross-references: KEGG:lcts

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.185838Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 393, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:13.922567Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:20.690187Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:47.523841Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007297
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.758900+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Minimal chemically-defined medium + His +Pro (letort 2001" to "minimal_chemically_defined_medium_his_pro_letort_2001" for consistent matching
2026-06-05T07:41:34.617177+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:02:54.559370+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-08-31T06:18:15.538004+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.