minimal_medium_jyssum_1959

CultureMech:007299 bacterial DEFINED LIQUID
Original name: Minimal medium (Jyssum 1959)

Overview

External ontology
MEDIADB:395 (source link unavailable) — Minimal medium (Jyssum 1959)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007299["minimal_medium_jyssum_1959"]
    ing0_Sodium_thiosulfate["Sodium thiosulfate"]
    CultureMech_007299 --> ing0_Sodium_thiosulfate
    CHEBI_132112["CHEBI:132112"]
    ing0_Sodium_thiosulfate -.-> CHEBI_132112
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007299 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007299 --> ing2_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing2_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing3_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007299 --> ing3_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing3_Ferrous_sulfate -.-> CHEBI_75832
    ing4_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007299 --> ing4_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing4_Ammonium_chloride -.-> CHEBI_31206
    ing5_Glucose["Glucose"]
    CultureMech_007299 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007299 --> ing6_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing6_Manganese_sulfate -.-> CHEBI_86364
    ing7_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007299 --> ing7_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing7_Magnesium_sulfate -.-> CHEBI_32599
    ing8_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007299 --> ing8_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing8_Potassium_dihydrogen_phosphate -.-> CHEBI_63036

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Sodium thiosulfate
2Na.O3S2 158.111 g/mol
0.1581 MILLIMOLAR CHEBI:132112
sodium thiosulfate
2 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.7752 MILLIMOLAR CHEBI:3312
calcium dichloride
3 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
40.05 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
4 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.006583 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
5 Ammonium chloride
Cl.H4N 53.492 g/mol
28.04 MILLIMOLAR CHEBI:31206
ammonium chloride
6 Glucose
C6H12O6 180.156 g/mol
5.551 MILLIMOLAR CHEBI:17234
glucose
7 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.006623 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
8 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.4057 MILLIMOLAR CHEBI:32599
magnesium sulfate
9 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
14.7 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.204102Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 395, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:47.537025Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007299
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.809974+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'Minimal medium (Jyssum 1959" to "minimal_medium_jyssum_1959" for consistent matching
2026-06-05T07:41:34.684282+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:18:15.589708+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.