bl_medium

CultureMech:007302 bacterial DEFINED LIQUID
Original name: BL Medium

Overview

External ontology
MEDIADB:401 (source link unavailable) — BL Medium
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007302["bl_medium"]
    ing0_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007302 --> ing0_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing0_Ammonium_chloride -.-> CHEBI_31206
    ing1_L_Valine["L-Valine"]
    CultureMech_007302 --> ing1_L_Valine
    CHEBI_16414["CHEBI:16414"]
    ing1_L_Valine -.-> CHEBI_16414
    ing2_L_Methionine["L-Methionine"]
    CultureMech_007302 --> ing2_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing2_L_Methionine -.-> CHEBI_16643
    ing3_L_Leucine["L-Leucine"]
    CultureMech_007302 --> ing3_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing3_L_Leucine -.-> CHEBI_15603
    ing4_L_Histidine["L-Histidine"]
    CultureMech_007302 --> ing4_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing4_L_Histidine -.-> CHEBI_15971
    ing5_L_Glutamine["L-Glutamine"]
    CultureMech_007302 --> ing5_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing5_L_Glutamine -.-> CHEBI_18050
    ing6_L_Glutamate["L-Glutamate"]
    CultureMech_007302 --> ing6_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing6_L_Glutamate -.-> CHEBI_16015
    ing7_Pantothenate["Pantothenate"]
    CultureMech_007302 --> ing7_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing7_Pantothenate -.-> CHEBI_16454
    ing8_Boric_acid["Boric acid"]
    CultureMech_007302 --> ing8_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing8_Boric_acid -.-> CHEBI_33118
    ing9_Riboflavin["Riboflavin"]
    CultureMech_007302 --> ing9_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing9_Riboflavin -.-> CHEBI_17015
    ing10_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007302 --> ing10_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing10_Thiamine_HCl -.-> CHEBI_49105
    ing11_Nicotinamide["Nicotinamide"]
    CultureMech_007302 --> ing11_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing11_Nicotinamide -.-> CHEBI_17154
    ing12_L_Asparagine["L-Asparagine"]
    CultureMech_007302 --> ing12_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing12_L_Asparagine -.-> CHEBI_17196
    ing13_L_Isoleucine["L-Isoleucine"]
    CultureMech_007302 --> ing13_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing13_L_Isoleucine -.-> CHEBI_17191
    ing14_Potassium_sulfate["Potassium sulfate"]
    CultureMech_007302 --> ing14_Potassium_sulfate
    CHEBI_32036["CHEBI:32036"]
    ing14_Potassium_sulfate -.-> CHEBI_32036
    ing15_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007302 --> ing15_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing15_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing16_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
    CultureMech_007302 --> ing16_Molybdic_acid_ammonium_salt_tetrahydrate
    CHEBI_91249["CHEBI:91249"]
    ing16_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
    ing17_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007302 --> ing17_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing17_Cobalt_chloride -.-> CHEBI_35696
    ing18_Sodium_acetate["Sodium acetate"]
    CultureMech_007302 --> ing18_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing18_Sodium_acetate -.-> CHEBI_32954
    ing19_Glucose["Glucose"]
    CultureMech_007302 --> ing19_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing19_Glucose -.-> CHEBI_17234
    ing20_MOPS["MOPS"]
    CultureMech_007302 --> ing20_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing20_MOPS -.-> CHEBI_39074
    ing21_Tricine["Tricine"]
    CultureMech_007302 --> ing21_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing21_Tricine -.-> CHEBI_46760
    ing22_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007302 --> ing22_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing22_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing23_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007302 --> ing23_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing23_Magnesium_chloride -.-> CHEBI_86345
    ing24_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007302 --> ing24_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing24_Ferrous_sulfate -.-> CHEBI_75832
    ing25_Sodium_chloride["Sodium chloride"]
    CultureMech_007302 --> ing25_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing25_Sodium_chloride -.-> CHEBI_26710
    ing26_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007302 --> ing26_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing26_Cupric_sulfate -.-> CHEBI_23414
    ing27_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007302 --> ing27_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing27_Zinc_sulfate -.-> CHEBI_35176
    ing28_Folate["Folate"]
    CultureMech_007302 --> ing28_Folate
    CHEBI_27470["CHEBI:27470"]
    ing28_Folate -.-> CHEBI_27470
    ing29_Biotin["Biotin"]
    CultureMech_007302 --> ing29_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing29_Biotin -.-> CHEBI_15956
    CultureMech_007302 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 Ammonium chloride
Cl.H4N 53.492 g/mol
9.5 MILLIMOLAR CHEBI:31206
ammonium chloride
2 L-Valine
C5H11NO2 117.148 g/mol
2.6 MILLIMOLAR CHEBI:16414
L-valine
 Cross-references: KEGG:val-L
3 L-Methionine
C5H11NO2S 149.215 g/mol
0.5 MILLIMOLAR CHEBI:16643
L-methionine
 Cross-references: KEGG:met-L
4 L-Leucine
C6H13NO2 131.175 g/mol
1.5 MILLIMOLAR CHEBI:15603
L-leucine
 Cross-references: KEGG:leu-L
5 L-Histidine
C6H9N3O2 155.157 g/mol
0.3 MILLIMOLAR CHEBI:15971
L-histidine
 Cross-references: KEGG:his-L
6 L-Glutamine
C5H10N2O3 146.146 g/mol
0.7 MILLIMOLAR CHEBI:18050
L-glutamine
 Cross-references: KEGG:gln-L
7 L-Glutamate
C5H9NO4 147.13 g/mol
21.0 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
8 Pantothenate
C9H16NO5 218.229 g/mol
0.002 MILLIMOLAR CHEBI:16454
pantothenate
 Cross-references: KEGG:pnto-R
9 Boric acid
H3BO3 61.833 g/mol
0.0004 MILLIMOLAR CHEBI:33118
boric acid
10 Riboflavin
C17H20N4O6 376.369 g/mol
0.0026 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
11 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.003 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
12 Nicotinamide
C6H6N2O 122.127 g/mol
0.008 MILLIMOLAR CHEBI:17154
nicotinamide
 Cross-references: KEGG:ncam
13 L-Asparagine
C4H8N2O3 132.119 g/mol
0.8 MILLIMOLAR CHEBI:17196
L-asparagine
 Cross-references: KEGG:asn-L
14 L-Isoleucine
C6H13NO2 131.175 g/mol
0.8 MILLIMOLAR CHEBI:17191
L-isoleucine
 Cross-references: KEGG:ile-L
15 Potassium sulfate
2K.O4S 174.259 g/mol
0.28 MILLIMOLAR CHEBI:32036
potassium sulfate
16 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
1.3 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
17 Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
3e-06 MILLIMOLAR CHEBI:91249
ammonium molybdate
18 Cobalt chloride
2Cl.Co 129.839 g/mol
3e-05 MILLIMOLAR CHEBI:35696
cobalt dichloride
19 Sodium acetate
C2H3O2.Na 82.034 g/mol
15.0 MILLIMOLAR CHEBI:32954
sodium acetate
20 Glucose
C6H12O6 180.156 g/mol
50.0 MILLIMOLAR CHEBI:17234
glucose
21 MOPS 190.0 MILLIMOLAR CHEBI:39074
MOPS
22 Tricine 4.0 MILLIMOLAR CHEBI:46760
tricine
23 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.0005 MILLIMOLAR CHEBI:3312
calcium dichloride
24 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.52 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
25 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.01 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
26 Sodium chloride
Cl.Na 58.443 g/mol
50.0 MILLIMOLAR CHEBI:26710
sodium chloride
27 Cupric sulfate
Cu.O4S 159.609 g/mol
1e-05 MILLIMOLAR CHEBI:23414
copper(II) sulfate
28 Zinc sulfate
O4S.Zn 161.453 g/mol
1e-05 MILLIMOLAR CHEBI:35176
zinc sulfate
29 Folate
C19H19N7O6 441.404 g/mol
0.0023 MILLIMOLAR CHEBI:27470
folic acid
 Cross-references: KEGG:fol
30 Biotin
C10H16N2O3S 244.316 g/mol
0.0004 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
31 Manganese chloride
Cl2Mn 125.844 g/mol
8e-05 MILLIMOLAR CHEBI:63041
manganese(II) chloride
32 Pyridoxal HCl
C8H9NO3.HCl 203.625 g/mol
0.01 MILLIMOLAR CHEBI:131529
pyridoxal hydrochloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.266337Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 401, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:32.363351Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:47.564962Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007302
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.844497+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BL Medium" to "bl_medium" for consistent matching
2026-06-05T07:40:51.029730+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.