bl_medium_asp

CultureMech:007304 bacterial DEFINED LIQUID
Original name: BL Medium + Asp

Overview

External ontology
MEDIADB:403 (source link unavailable) — BL Medium + Asp
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007304["bl_medium_asp"]
    ing0_L_Leucine["L-Leucine"]
    CultureMech_007304 --> ing0_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing0_L_Leucine -.-> CHEBI_15603
    ing1_Potassium_sulfate["Potassium sulfate"]
    CultureMech_007304 --> ing1_Potassium_sulfate
    CHEBI_32036["CHEBI:32036"]
    ing1_Potassium_sulfate -.-> CHEBI_32036
    ing2_Sodium_chloride["Sodium chloride"]
    CultureMech_007304 --> ing2_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_chloride -.-> CHEBI_26710
    ing3_L_Isoleucine["L-Isoleucine"]
    CultureMech_007304 --> ing3_L_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing3_L_Isoleucine -.-> CHEBI_17191
    ing4_Folate["Folate"]
    CultureMech_007304 --> ing4_Folate
    CHEBI_27470["CHEBI:27470"]
    ing4_Folate -.-> CHEBI_27470
    ing5_Pyridoxal_HCl["Pyridoxal HCl"]
    CultureMech_007304 --> ing5_Pyridoxal_HCl
    CHEBI_131529["CHEBI:131529"]
    ing5_Pyridoxal_HCl -.-> CHEBI_131529
    ing6_L_Glutamine["L-Glutamine"]
    CultureMech_007304 --> ing6_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing6_L_Glutamine -.-> CHEBI_18050
    ing7_Tricine["Tricine"]
    CultureMech_007304 --> ing7_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing7_Tricine -.-> CHEBI_46760
    ing8_L_Aspartate["L-Aspartate"]
    CultureMech_007304 --> ing8_L_Aspartate
    CHEBI_29991["CHEBI:29991"]
    ing8_L_Aspartate -.-> CHEBI_29991
    ing9_L_Histidine["L-Histidine"]
    CultureMech_007304 --> ing9_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing9_L_Histidine -.-> CHEBI_15971
    ing10_L_Methionine["L-Methionine"]
    CultureMech_007304 --> ing10_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing10_L_Methionine -.-> CHEBI_16643
    ing11_L_Glutamate["L-Glutamate"]
    CultureMech_007304 --> ing11_L_Glutamate
    CHEBI_16015["CHEBI:16015"]
    ing11_L_Glutamate -.-> CHEBI_16015
    ing12_L_Asparagine["L-Asparagine"]
    CultureMech_007304 --> ing12_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing12_L_Asparagine -.-> CHEBI_17196
    ing13_Biotin["Biotin"]
    CultureMech_007304 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Nicotinamide["Nicotinamide"]
    CultureMech_007304 --> ing14_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing14_Nicotinamide -.-> CHEBI_17154
    ing15_Cupric_sulfate["Cupric sulfate"]
    CultureMech_007304 --> ing15_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing15_Cupric_sulfate -.-> CHEBI_23414
    ing16_MOPS["MOPS"]
    CultureMech_007304 --> ing16_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing16_MOPS -.-> CHEBI_39074
    ing17_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007304 --> ing17_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing17_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing18_Thiamine_HCl["Thiamine HCl"]
    CultureMech_007304 --> ing18_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing18_Thiamine_HCl -.-> CHEBI_49105
    ing19_Glucose["Glucose"]
    CultureMech_007304 --> ing19_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing19_Glucose -.-> CHEBI_17234
    ing20_Riboflavin["Riboflavin"]
    CultureMech_007304 --> ing20_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavin -.-> CHEBI_17015
    ing21_L_Valine["L-Valine"]
    CultureMech_007304 --> ing21_L_Valine
    CHEBI_16414["CHEBI:16414"]
    ing21_L_Valine -.-> CHEBI_16414
    ing22_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007304 --> ing22_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing22_Ferrous_sulfate -.-> CHEBI_75832
    ing23_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007304 --> ing23_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing23_Cobalt_chloride -.-> CHEBI_35696
    ing24_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007304 --> ing24_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing24_Zinc_sulfate -.-> CHEBI_35176
    ing25_Manganese_chloride["Manganese chloride"]
    CultureMech_007304 --> ing25_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing25_Manganese_chloride -.-> CHEBI_63041
    ing26_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007304 --> ing26_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing26_Magnesium_chloride -.-> CHEBI_86345
    ing27_Pantothenate["Pantothenate"]
    CultureMech_007304 --> ing27_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing27_Pantothenate -.-> CHEBI_16454
    ing28_Boric_acid["Boric acid"]
    CultureMech_007304 --> ing28_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing28_Boric_acid -.-> CHEBI_33118
    ing29_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007304 --> ing29_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing29_Calcium_chloride_anhydrous -.-> CHEBI_3312
    CultureMech_007304 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 L-Leucine
C6H13NO2 131.175 g/mol
1.5 MILLIMOLAR CHEBI:15603
L-leucine
 Cross-references: KEGG:leu-L
2 Potassium sulfate
2K.O4S 174.259 g/mol
0.28 MILLIMOLAR CHEBI:32036
potassium sulfate
3 Sodium chloride
Cl.Na 58.443 g/mol
50.0 MILLIMOLAR CHEBI:26710
sodium chloride
4 L-Isoleucine
C6H13NO2 131.175 g/mol
0.8 MILLIMOLAR CHEBI:17191
L-isoleucine
 Cross-references: KEGG:ile-L
5 Folate
C19H19N7O6 441.404 g/mol
0.0023 MILLIMOLAR CHEBI:27470
folic acid
 Cross-references: KEGG:fol
6 Pyridoxal HCl
C8H9NO3.HCl 203.625 g/mol
0.01 MILLIMOLAR CHEBI:131529
pyridoxal hydrochloride
7 L-Glutamine
C5H10N2O3 146.146 g/mol
0.7 MILLIMOLAR CHEBI:18050
L-glutamine
 Cross-references: KEGG:gln-L
8 Tricine 4.0 MILLIMOLAR CHEBI:46760
tricine
9 L-Aspartate
C4H6NO4 132.095 g/mol
1.0 MILLIMOLAR CHEBI:29991
L-aspartate(1-)
 Cross-references: KEGG:asp-L
10 L-Histidine
C6H9N3O2 155.157 g/mol
0.3 MILLIMOLAR CHEBI:15971
L-histidine
 Cross-references: KEGG:his-L
11 L-Methionine
C5H11NO2S 149.215 g/mol
0.5 MILLIMOLAR CHEBI:16643
L-methionine
 Cross-references: KEGG:met-L
12 L-Glutamate
C5H9NO4 147.13 g/mol
21.0 MILLIMOLAR CHEBI:16015
L-glutamic acid
 Cross-references: KEGG:glu-L
13 L-Asparagine
C4H8N2O3 132.119 g/mol
0.8 MILLIMOLAR CHEBI:17196
L-asparagine
 Cross-references: KEGG:asn-L
14 Biotin
C10H16N2O3S 244.316 g/mol
0.0004 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
15 Nicotinamide
C6H6N2O 122.127 g/mol
0.008 MILLIMOLAR CHEBI:17154
nicotinamide
 Cross-references: KEGG:ncam
16 Cupric sulfate
Cu.O4S 159.609 g/mol
1e-05 MILLIMOLAR CHEBI:23414
copper(II) sulfate
17 MOPS 190.0 MILLIMOLAR CHEBI:39074
MOPS
18 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
1.3 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
19 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.003 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
20 Glucose
C6H12O6 180.156 g/mol
50.0 MILLIMOLAR CHEBI:17234
glucose
21 Riboflavin
C17H20N4O6 376.369 g/mol
0.0026 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
22 L-Valine
C5H11NO2 117.148 g/mol
2.6 MILLIMOLAR CHEBI:16414
L-valine
 Cross-references: KEGG:val-L
23 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.01 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
24 Cobalt chloride
2Cl.Co 129.839 g/mol
3e-05 MILLIMOLAR CHEBI:35696
cobalt dichloride
25 Zinc sulfate
O4S.Zn 161.453 g/mol
1e-05 MILLIMOLAR CHEBI:35176
zinc sulfate
26 Manganese chloride
Cl2Mn 125.844 g/mol
8e-05 MILLIMOLAR CHEBI:63041
manganese(II) chloride
27 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.52 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
28 Pantothenate
C9H16NO5 218.229 g/mol
0.002 MILLIMOLAR CHEBI:16454
pantothenate
 Cross-references: KEGG:pnto-R
29 Boric acid
H3BO3 61.833 g/mol
0.0004 MILLIMOLAR CHEBI:33118
boric acid
30 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.0005 MILLIMOLAR CHEBI:3312
calcium dichloride
31 Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
3e-06 MILLIMOLAR CHEBI:91249
ammonium molybdate
32 Ammonium chloride
Cl.H4N 53.492 g/mol
9.5 MILLIMOLAR CHEBI:31206
ammonium chloride
33 Sodium acetate
C2H3O2.Na 82.034 g/mol
15.0 MILLIMOLAR CHEBI:32954
sodium acetate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.287730Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 403, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:12.311814Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:47.592278Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007304
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.873777+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BL Medium + Asp" to "bl_medium_asp" for consistent matching
2026-06-05T07:40:51.082128+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:01:35.177667+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).