ms10r_medium

CultureMech:007309 bacterial DEFINED LIQUID
Original name: MS10R Medium

Overview

External ontology
MEDIADB:409 (source link unavailable) — MS10R Medium
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007309["ms10r_medium"]
    ing0_Tryptophan["Tryptophan"]
    CultureMech_007309 --> ing0_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing0_Tryptophan -.-> CHEBI_27897
    ing1_Glucose["Glucose"]
    CultureMech_007309 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Glycine["Glycine"]
    CultureMech_007309 --> ing2_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing2_Glycine -.-> CHEBI_15428
    ing3_Nicotinate["Nicotinate"]
    CultureMech_007309 --> ing3_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing3_Nicotinate -.-> CHEBI_15940
    ing4_Cysteine["Cysteine"]
    CultureMech_007309 --> ing4_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing4_Cysteine -.-> CHEBI_15356
    ing5_Glutamine["Glutamine"]
    CultureMech_007309 --> ing5_Glutamine
    ing6_Biotin["Biotin"]
    CultureMech_007309 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_DL_Serine["DL-Serine"]
    CultureMech_007309 --> ing7_DL_Serine
    ing8_Pyridoxamine["Pyridoxamine"]
    CultureMech_007309 --> ing8_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing8_Pyridoxamine -.-> CHEBI_16410
    ing9_Histidine["Histidine"]
    CultureMech_007309 --> ing9_Histidine
    CHEBI_27570["CHEBI:27570"]
    ing9_Histidine -.-> CHEBI_27570
    ing10_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007309 --> ing10_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing10_Calcium_pantothenate -.-> CHEBI_31345
    ing11_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007309 --> ing11_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing11_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing12_Threonine["Threonine"]
    CultureMech_007309 --> ing12_Threonine
    CHEBI_26986["CHEBI:26986"]
    ing12_Threonine -.-> CHEBI_26986
    ing13_Lysine_HCl["Lysine-HCl"]
    CultureMech_007309 --> ing13_Lysine_HCl
    ing14_DL_methionine["DL-methionine"]
    CultureMech_007309 --> ing14_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing14_DL_methionine -.-> CHEBI_16811
    ing15_Riboflavin["Riboflavin"]
    CultureMech_007309 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Leucine["Leucine"]
    CultureMech_007309 --> ing16_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing16_Leucine -.-> CHEBI_15603
    ing17_Proline["Proline"]
    CultureMech_007309 --> ing17_Proline
    CHEBI_26271["CHEBI:26271"]
    ing17_Proline -.-> CHEBI_26271
    ing18_Isoleucine["Isoleucine"]
    CultureMech_007309 --> ing18_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing18_Isoleucine -.-> CHEBI_17191
    ing19_DL_Tyrosine["DL-Tyrosine"]
    CultureMech_007309 --> ing19_DL_Tyrosine
    CHEBI_18186["CHEBI:18186"]
    ing19_DL_Tyrosine -.-> CHEBI_18186
    ing20_Alanine["Alanine"]
    CultureMech_007309 --> ing20_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing20_Alanine -.-> CHEBI_16977
    ing21_Arginine["Arginine"]
    CultureMech_007309 --> ing21_Arginine
    CHEBI_29016["CHEBI:29016"]
    ing21_Arginine -.-> CHEBI_29016
    ing22_Phenylalanine["Phenylalanine"]
    CultureMech_007309 --> ing22_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing22_Phenylalanine -.-> CHEBI_17295
    ing23_Asparagine["Asparagine"]
    CultureMech_007309 --> ing23_Asparagine
    CHEBI_22653["CHEBI:22653"]
    ing23_Asparagine -.-> CHEBI_22653
    ing24_Valine["Valine"]
    CultureMech_007309 --> ing24_Valine
    CHEBI_16414["CHEBI:16414"]
    ing24_Valine -.-> CHEBI_16414
    ing25_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007309 --> ing25_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing25_Ammonium_sulfate -.-> CHEBI_62946
    ing26_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007309 --> ing26_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing26_Magnesium_chloride -.-> CHEBI_86345
    ing27_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007309 --> ing27_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing27_Potassium_dibasic_phosphate -.-> CHEBI_131527

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Tryptophan
C11H12N2O2 204.229 g/mol
0.2448 MILLIMOLAR CHEBI:27897
tryptophan
2 Glucose
C6H12O6 180.156 g/mol
55.51 MILLIMOLAR CHEBI:17234
glucose
3 Glycine
C2H5NO2 75.067 g/mol
2.265 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
4 Nicotinate
C6H5NO2 123.111 g/mol
0.008123 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
5 Cysteine
C3H7NO2S 121.161 g/mol
1.403 MILLIMOLAR CHEBI:15356
cysteine
6 Glutamine 3.49 MILLIMOLAR
7 Biotin
C10H16N2O3S 244.316 g/mol
0.04093 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
8 DL-Serine 3.235 MILLIMOLAR
9 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.02973 MILLIMOLAR CHEBI:16410
pyridoxamine
 Cross-references: KEGG:pydam
10 Histidine
C6H9N3O2 155.157 g/mol
0.7089 MILLIMOLAR CHEBI:27570
histidine
11 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.002099 MILLIMOLAR CHEBI:31345
Calcium pantothenate
12 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
66.13 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
13 Threonine
C4H9NO3 119.119 g/mol
1.931 MILLIMOLAR CHEBI:26986
threonine
14 Lysine-HCl 1.916 MILLIMOLAR
15 DL-methionine
C5H11NO2S 149.215 g/mol
0.8042 MILLIMOLAR CHEBI:16811
methionine
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.002657 MILLIMOLAR CHEBI:17015
riboflavin
 Cross-references: KEGG:ribflv
17 Leucine
C6H13NO2 131.175 g/mol
3.583 MILLIMOLAR CHEBI:15603
L-leucine
18 Proline
C5H9NO2 115.132 g/mol
5.906 MILLIMOLAR CHEBI:26271
proline
19 Isoleucine
C6H13NO2 131.175 g/mol
1.525 MILLIMOLAR CHEBI:17191
L-isoleucine
20 DL-Tyrosine
C9H11NO3 181.191 g/mol
1.601 MILLIMOLAR CHEBI:18186
tyrosine
21 Alanine
C3H7NO2 89.094 g/mol
2.694 MILLIMOLAR CHEBI:16977
L-alanine
22 Arginine
C6H14N4O2 174.204 g/mol
0.6889 MILLIMOLAR CHEBI:29016
arginine
23 Phenylalanine
C9H11NO2 165.192 g/mol
1.695 MILLIMOLAR CHEBI:17295
L-phenylalanine
24 Asparagine
C4H8N2O3 132.119 g/mol
2.573 MILLIMOLAR CHEBI:22653
asparagine
25 Valine
C5H11NO2 117.148 g/mol
2.817 MILLIMOLAR CHEBI:16414
L-valine
26 Ammonium sulfate
2H4N.O4S 132.141 g/mol
1.362 MILLIMOLAR CHEBI:62946
ammonium sulfate
27 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.9838 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
28 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
42.92 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.341425Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 409, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:15.532927Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:22.555707Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:47.661513Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007309
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.948301+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MS10R Medium" to "ms10r_medium" for consistent matching
2026-06-05T07:41:38.636023+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:02:58.924496+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).