ms10_medium_supplements_for_continuous_cultivation

CultureMech:007313 bacterial DEFINED LIQUID
Original name: MS10 Medium + Supplements for Continuous Cultivation

Overview

External ontology
MEDIADB:412 (source link unavailable) — MS10 Medium + Supplements for Continuous Cultivation
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007313["ms10_medium_supplements_for_continuous_cultivation"]
    ing0_Tryptophan["Tryptophan"]
    CultureMech_007313 --> ing0_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing0_Tryptophan -.-> CHEBI_27897
    ing1_Manganese_chloride["Manganese chloride"]
    CultureMech_007313 --> ing1_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing1_Manganese_chloride -.-> CHEBI_63041
    ing2_Glucose["Glucose"]
    CultureMech_007313 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Glycine["Glycine"]
    CultureMech_007313 --> ing3_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing3_Glycine -.-> CHEBI_15428
    ing4_Nicotinate["Nicotinate"]
    CultureMech_007313 --> ing4_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing4_Nicotinate -.-> CHEBI_15940
    ing5_Cysteine["Cysteine"]
    CultureMech_007313 --> ing5_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing5_Cysteine -.-> CHEBI_15356
    ing6_Glutamine["Glutamine"]
    CultureMech_007313 --> ing6_Glutamine
    ing7_Biotin["Biotin"]
    CultureMech_007313 --> ing7_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing7_Biotin -.-> CHEBI_15956
    ing8_DL_Serine["DL-Serine"]
    CultureMech_007313 --> ing8_DL_Serine
    ing9_Pyridoxamine["Pyridoxamine"]
    CultureMech_007313 --> ing9_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing9_Pyridoxamine -.-> CHEBI_16410
    ing10_Histidine["Histidine"]
    CultureMech_007313 --> ing10_Histidine
    CHEBI_27570["CHEBI:27570"]
    ing10_Histidine -.-> CHEBI_27570
    ing11_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007313 --> ing11_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing11_Calcium_pantothenate -.-> CHEBI_31345
    ing12_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007313 --> ing12_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing12_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing13_Threonine["Threonine"]
    CultureMech_007313 --> ing13_Threonine
    CHEBI_26986["CHEBI:26986"]
    ing13_Threonine -.-> CHEBI_26986
    ing14_Lysine_HCl["Lysine-HCl"]
    CultureMech_007313 --> ing14_Lysine_HCl
    ing15_DL_methionine["DL-methionine"]
    CultureMech_007313 --> ing15_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing15_DL_methionine -.-> CHEBI_16811
    ing16_Thiamine["Thiamine"]
    CultureMech_007313 --> ing16_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing16_Thiamine -.-> CHEBI_18385
    ing17_Lipoate["Lipoate"]
    CultureMech_007313 --> ing17_Lipoate
    CHEBI_16494["CHEBI:16494"]
    ing17_Lipoate -.-> CHEBI_16494
    ing18_Leucine["Leucine"]
    CultureMech_007313 --> ing18_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing18_Leucine -.-> CHEBI_15603
    ing19_Proline["Proline"]
    CultureMech_007313 --> ing19_Proline
    CHEBI_26271["CHEBI:26271"]
    ing19_Proline -.-> CHEBI_26271
    ing20_Isoleucine["Isoleucine"]
    CultureMech_007313 --> ing20_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing20_Isoleucine -.-> CHEBI_17191
    ing21_DL_Tyrosine["DL-Tyrosine"]
    CultureMech_007313 --> ing21_DL_Tyrosine
    CHEBI_18186["CHEBI:18186"]
    ing21_DL_Tyrosine -.-> CHEBI_18186
    ing22_Alanine["Alanine"]
    CultureMech_007313 --> ing22_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing22_Alanine -.-> CHEBI_16977
    ing23_Arginine["Arginine"]
    CultureMech_007313 --> ing23_Arginine
    CHEBI_29016["CHEBI:29016"]
    ing23_Arginine -.-> CHEBI_29016
    ing24_Phenylalanine["Phenylalanine"]
    CultureMech_007313 --> ing24_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing24_Phenylalanine -.-> CHEBI_17295
    ing25_Asparagine["Asparagine"]
    CultureMech_007313 --> ing25_Asparagine
    CHEBI_22653["CHEBI:22653"]
    ing25_Asparagine -.-> CHEBI_22653
    ing26_Valine["Valine"]
    CultureMech_007313 --> ing26_Valine
    CHEBI_16414["CHEBI:16414"]
    ing26_Valine -.-> CHEBI_16414
    ing27_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007313 --> ing27_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing27_Ammonium_sulfate -.-> CHEBI_62946
    ing28_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007313 --> ing28_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing28_Magnesium_chloride -.-> CHEBI_86345
    ing29_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007313 --> ing29_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing29_Potassium_dibasic_phosphate -.-> CHEBI_131527

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 Tryptophan
C11H12N2O2 204.229 g/mol
0.2448 MILLIMOLAR CHEBI:27897
tryptophan
2 Manganese chloride
Cl2Mn 125.844 g/mol
9.933e-05 MILLIMOLAR CHEBI:63041
manganese(II) chloride
3 Glucose
C6H12O6 180.156 g/mol
16.65 MILLIMOLAR CHEBI:17234
glucose
4 Glycine
C2H5NO2 75.067 g/mol
2.265 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
5 Nicotinate
C6H5NO2 123.111 g/mol
0.008123 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
6 Cysteine
C3H7NO2S 121.161 g/mol
1.403 MILLIMOLAR CHEBI:15356
cysteine
7 Glutamine 3.49 MILLIMOLAR
8 Biotin
C10H16N2O3S 244.316 g/mol
0.04093 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
9 DL-Serine 3.235 MILLIMOLAR
10 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.02973 MILLIMOLAR CHEBI:16410
pyridoxamine
 Cross-references: KEGG:pydam
11 Histidine
C6H9N3O2 155.157 g/mol
0.7089 MILLIMOLAR CHEBI:27570
histidine
12 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.002099 MILLIMOLAR CHEBI:31345
Calcium pantothenate
13 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
66.13 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
14 Threonine
C4H9NO3 119.119 g/mol
1.931 MILLIMOLAR CHEBI:26986
threonine
15 Lysine-HCl 1.916 MILLIMOLAR
16 DL-methionine
C5H11NO2S 149.215 g/mol
0.8042 MILLIMOLAR CHEBI:16811
methionine
17 Thiamine
C12H17N4OS 265.362 g/mol
0.003769 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
18 Lipoate
C8H14O2S2 206.332 g/mol
0.01212 MILLIMOLAR CHEBI:16494
lipoic acid
 Cross-references: KEGG:lipoate
19 Leucine
C6H13NO2 131.175 g/mol
3.583 MILLIMOLAR CHEBI:15603
L-leucine
20 Proline
C5H9NO2 115.132 g/mol
5.906 MILLIMOLAR CHEBI:26271
proline
21 Isoleucine
C6H13NO2 131.175 g/mol
1.525 MILLIMOLAR CHEBI:17191
L-isoleucine
22 DL-Tyrosine
C9H11NO3 181.191 g/mol
1.601 MILLIMOLAR CHEBI:18186
tyrosine
23 Alanine
C3H7NO2 89.094 g/mol
2.694 MILLIMOLAR CHEBI:16977
L-alanine
24 Arginine
C6H14N4O2 174.204 g/mol
0.6889 MILLIMOLAR CHEBI:29016
arginine
25 Phenylalanine
C9H11NO2 165.192 g/mol
1.695 MILLIMOLAR CHEBI:17295
L-phenylalanine
26 Asparagine
C4H8N2O3 132.119 g/mol
2.573 MILLIMOLAR CHEBI:22653
asparagine
27 Valine
C5H11NO2 117.148 g/mol
2.817 MILLIMOLAR CHEBI:16414
L-valine
28 Ammonium sulfate
2H4N.O4S 132.141 g/mol
1.362 MILLIMOLAR CHEBI:62946
ammonium sulfate
29 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.9838 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
30 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
42.92 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.371747Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 412, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:37.012931Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:48.147059Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:47.694790Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007313
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:05.983842+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MS10 Medium + Supplements for Continuous Cultivation" to "ms10_medium_supplements_for_continuous_cultivation" for consistent matching
2026-06-05T07:41:38.621149+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:02:58.891731+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).