ms10_medium_supplements_acetate_for_batch_cultivation

CultureMech:007315 bacterial DEFINED LIQUID
Original name: MS10 Medium + Supplements + Acetate for Batch Cultivation

Overview

External ontology
MEDIADB:414 (source link unavailable) — MS10 Medium + Supplements + Acetate for Batch Cultivation
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007315["ms10_medium_supplements_acetate_for_batch_cultivation"]
    ing0_Cysteine["Cysteine"]
    CultureMech_007315 --> ing0_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing0_Cysteine -.-> CHEBI_15356
    ing1_Proline["Proline"]
    CultureMech_007315 --> ing1_Proline
    CHEBI_26271["CHEBI:26271"]
    ing1_Proline -.-> CHEBI_26271
    ing2_Sodium_acetate["Sodium acetate"]
    CultureMech_007315 --> ing2_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing2_Sodium_acetate -.-> CHEBI_32954
    ing3_Glucose["Glucose"]
    CultureMech_007315 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Glycine["Glycine"]
    CultureMech_007315 --> ing4_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing4_Glycine -.-> CHEBI_15428
    ing5_Nicotinate["Nicotinate"]
    CultureMech_007315 --> ing5_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing5_Nicotinate -.-> CHEBI_15940
    ing6_DL_Serine["DL-Serine"]
    CultureMech_007315 --> ing6_DL_Serine
    ing7_Glutamine["Glutamine"]
    CultureMech_007315 --> ing7_Glutamine
    ing8_Biotin["Biotin"]
    CultureMech_007315 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Pyridoxamine["Pyridoxamine"]
    CultureMech_007315 --> ing9_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing9_Pyridoxamine -.-> CHEBI_16410
    ing10_Thiamine["Thiamine"]
    CultureMech_007315 --> ing10_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing10_Thiamine -.-> CHEBI_18385
    ing11_Lipoate["Lipoate"]
    CultureMech_007315 --> ing11_Lipoate
    CHEBI_16494["CHEBI:16494"]
    ing11_Lipoate -.-> CHEBI_16494
    ing12_DL_methionine["DL-methionine"]
    CultureMech_007315 --> ing12_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing12_DL_methionine -.-> CHEBI_16811
    ing13_Valine["Valine"]
    CultureMech_007315 --> ing13_Valine
    CHEBI_16414["CHEBI:16414"]
    ing13_Valine -.-> CHEBI_16414
    ing14_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_007315 --> ing14_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing14_Calcium_pantothenate -.-> CHEBI_31345
    ing15_Leucine["Leucine"]
    CultureMech_007315 --> ing15_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing15_Leucine -.-> CHEBI_15603
    ing16_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007315 --> ing16_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing16_Ammonium_sulfate -.-> CHEBI_62946
    ing17_Isoleucine["Isoleucine"]
    CultureMech_007315 --> ing17_Isoleucine
    CHEBI_17191["CHEBI:17191"]
    ing17_Isoleucine -.-> CHEBI_17191
    ing18_DL_Tyrosine["DL-Tyrosine"]
    CultureMech_007315 --> ing18_DL_Tyrosine
    CHEBI_18186["CHEBI:18186"]
    ing18_DL_Tyrosine -.-> CHEBI_18186
    ing19_Threonine["Threonine"]
    CultureMech_007315 --> ing19_Threonine
    CHEBI_26986["CHEBI:26986"]
    ing19_Threonine -.-> CHEBI_26986
    ing20_Phenylalanine["Phenylalanine"]
    CultureMech_007315 --> ing20_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing20_Phenylalanine -.-> CHEBI_17295
    ing21_Manganese_chloride["Manganese chloride"]
    CultureMech_007315 --> ing21_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing21_Manganese_chloride -.-> CHEBI_63041
    ing22_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007315 --> ing22_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing22_Magnesium_chloride -.-> CHEBI_86345
    ing23_Arginine["Arginine"]
    CultureMech_007315 --> ing23_Arginine
    CHEBI_29016["CHEBI:29016"]
    ing23_Arginine -.-> CHEBI_29016
    ing24_Histidine["Histidine"]
    CultureMech_007315 --> ing24_Histidine
    CHEBI_27570["CHEBI:27570"]
    ing24_Histidine -.-> CHEBI_27570
    ing25_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007315 --> ing25_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing25_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing26_Alanine["Alanine"]
    CultureMech_007315 --> ing26_Alanine
    CHEBI_16977["CHEBI:16977"]
    ing26_Alanine -.-> CHEBI_16977
    ing27_Tryptophan["Tryptophan"]
    CultureMech_007315 --> ing27_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing27_Tryptophan -.-> CHEBI_27897
    ing28_Asparagine["Asparagine"]
    CultureMech_007315 --> ing28_Asparagine
    CHEBI_22653["CHEBI:22653"]
    ing28_Asparagine -.-> CHEBI_22653
    ing29_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007315 --> ing29_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing29_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    CultureMech_007315 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 Cysteine
C3H7NO2S 121.161 g/mol
1.403 MILLIMOLAR CHEBI:15356
cysteine
2 Proline
C5H9NO2 115.132 g/mol
5.906 MILLIMOLAR CHEBI:26271
proline
3 Sodium acetate
C2H3O2.Na 82.034 g/mol
3.657 MILLIMOLAR CHEBI:32954
sodium acetate
4 Glucose
C6H12O6 180.156 g/mol
55.51 MILLIMOLAR CHEBI:17234
glucose
5 Glycine
C2H5NO2 75.067 g/mol
2.265 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
6 Nicotinate
C6H5NO2 123.111 g/mol
0.008123 MILLIMOLAR CHEBI:15940
nicotinic acid
 Cross-references: KEGG:nac
7 DL-Serine 3.235 MILLIMOLAR
8 Glutamine 3.49 MILLIMOLAR
9 Biotin
C10H16N2O3S 244.316 g/mol
0.04093 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
10 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.02973 MILLIMOLAR CHEBI:16410
pyridoxamine
 Cross-references: KEGG:pydam
11 Thiamine
C12H17N4OS 265.362 g/mol
0.003769 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
12 Lipoate
C8H14O2S2 206.332 g/mol
0.01212 MILLIMOLAR CHEBI:16494
lipoic acid
 Cross-references: KEGG:lipoate
13 DL-methionine
C5H11NO2S 149.215 g/mol
0.8042 MILLIMOLAR CHEBI:16811
methionine
14 Valine
C5H11NO2 117.148 g/mol
2.817 MILLIMOLAR CHEBI:16414
L-valine
15 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.002099 MILLIMOLAR CHEBI:31345
Calcium pantothenate
16 Leucine
C6H13NO2 131.175 g/mol
3.583 MILLIMOLAR CHEBI:15603
L-leucine
17 Ammonium sulfate
2H4N.O4S 132.141 g/mol
1.362 MILLIMOLAR CHEBI:62946
ammonium sulfate
18 Isoleucine
C6H13NO2 131.175 g/mol
1.525 MILLIMOLAR CHEBI:17191
L-isoleucine
19 DL-Tyrosine
C9H11NO3 181.191 g/mol
1.601 MILLIMOLAR CHEBI:18186
tyrosine
20 Threonine
C4H9NO3 119.119 g/mol
1.931 MILLIMOLAR CHEBI:26986
threonine
21 Phenylalanine
C9H11NO2 165.192 g/mol
1.695 MILLIMOLAR CHEBI:17295
L-phenylalanine
22 Manganese chloride
Cl2Mn 125.844 g/mol
9.933e-05 MILLIMOLAR CHEBI:63041
manganese(II) chloride
23 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.9838 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
24 Arginine
C6H14N4O2 174.204 g/mol
0.6889 MILLIMOLAR CHEBI:29016
arginine
25 Histidine
C6H9N3O2 155.157 g/mol
0.7089 MILLIMOLAR CHEBI:27570
histidine
26 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
42.92 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
27 Alanine
C3H7NO2 89.094 g/mol
2.694 MILLIMOLAR CHEBI:16977
L-alanine
28 Tryptophan
C11H12N2O2 204.229 g/mol
0.2448 MILLIMOLAR CHEBI:27897
tryptophan
29 Asparagine
C4H8N2O3 132.119 g/mol
2.573 MILLIMOLAR CHEBI:22653
asparagine
30 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
66.13 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
31 Lysine-HCl 1.916 MILLIMOLAR

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:46.394993Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 414, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:28.577031Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:37.966979Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:47.719369Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007315
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:06.010507+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MS10 Medium + Supplements + Acetate for Batch Cultivation" to "ms10_medium_supplements_acetate_for_batch_cultivation" for consistent matching
2026-06-05T07:41:38.574680+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-20T19:02:58.773156+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).