ams1_pyruvate_glycine

CultureMech:007391 bacterial DEFINED LIQUID
Original name: AMS1 (Pyruvate+Glycine) + Thiamin + 2-aminoethylphosphonic acid

Overview

External ontology
MEDIADB:483 (source link unavailable) — AMS1 (Pyruvate+Glycine) + Thiamin + 2-aminoethylphosphonic acid
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
High-metal flag
True
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007391["ams1_pyruvate_glycine"]
    ing0_Biotin["Biotin"]
    CultureMech_007391 --> ing0_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing0_Biotin -.-> CHEBI_15956
    ing1_Thiamine["Thiamine"]
    CultureMech_007391 --> ing1_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing1_Thiamine -.-> CHEBI_18385
    ing2_Pyruvate["Pyruvate"]
    CultureMech_007391 --> ing2_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing2_Pyruvate -.-> CHEBI_15361
    ing3_Nickel_chloride["Nickel chloride"]
    CultureMech_007391 --> ing3_Nickel_chloride
    CHEBI_34887["CHEBI:34887"]
    ing3_Nickel_chloride -.-> CHEBI_34887
    ing4_Magnesium_chloride["Magnesium chloride"]
    CultureMech_007391 --> ing4_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing4_Magnesium_chloride -.-> CHEBI_86345
    ing5_Glycine["Glycine"]
    CultureMech_007391 --> ing5_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing5_Glycine -.-> CHEBI_15428
    ing6_Sodium_bicarbonate["Sodium bicarbonate"]
    CultureMech_007391 --> ing6_Sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing6_Sodium_bicarbonate -.-> CHEBI_32139
    ing7_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007391 --> ing7_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing7_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing8_Sodium_molybdate["Sodium molybdate"]
    CultureMech_007391 --> ing8_Sodium_molybdate
    CHEBI_75215["CHEBI:75215"]
    ing8_Sodium_molybdate -.-> CHEBI_75215
    ing9_Iron_III__chloride["Iron(III) chloride"]
    CultureMech_007391 --> ing9_Iron_III__chloride
    ing10_Zinc_sulfate["Zinc sulfate"]
    CultureMech_007391 --> ing10_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing10_Zinc_sulfate -.-> CHEBI_35176
    ing11_Manganese_chloride["Manganese chloride"]
    CultureMech_007391 --> ing11_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing11_Manganese_chloride -.-> CHEBI_63041
    ing12_2_Aminoethylphosphonate["2-Aminoethylphosphonate"]
    CultureMech_007391 --> ing12_2_Aminoethylphosphonate
    ing13_Potassium_chloride["Potassium chloride"]
    CultureMech_007391 --> ing13_Potassium_chloride
    CHEBI_32588["CHEBI:32588"]
    ing13_Potassium_chloride -.-> CHEBI_32588
    ing14_DL_methionine["DL-methionine"]
    CultureMech_007391 --> ing14_DL_methionine
    CHEBI_16811["CHEBI:16811"]
    ing14_DL_methionine -.-> CHEBI_16811
    ing15_Cobalt_chloride["Cobalt chloride"]
    CultureMech_007391 --> ing15_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing15_Cobalt_chloride -.-> CHEBI_35696
    ing16_Sodium_chloride["Sodium chloride"]
    CultureMech_007391 --> ing16_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing16_Sodium_chloride -.-> CHEBI_26710
    ing17_Ammonium_sulfate["Ammonium sulfate"]
    CultureMech_007391 --> ing17_Ammonium_sulfate
    CHEBI_62946["CHEBI:62946"]
    ing17_Ammonium_sulfate -.-> CHEBI_62946
    ing18_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007391 --> ing18_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing18_Magnesium_sulfate -.-> CHEBI_32599
    ing19_Sodium_selenite["Sodium selenite"]
    CultureMech_007391 --> ing19_Sodium_selenite
    CHEBI_48843["CHEBI:48843"]
    ing19_Sodium_selenite -.-> CHEBI_48843

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Biotin
C10H16N2O3S 244.316 g/mol
4e-06 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
2 Thiamine
C12H17N4OS 265.362 g/mol
0.06 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
3 Pyruvate
C3H3O3 87.054 g/mol
1.0 MILLIMOLAR CHEBI:15361
pyruvate
 Cross-references: KEGG:pyr
4 Nickel chloride
Cl2Ni 129.599 g/mol
1e-06 MILLIMOLAR CHEBI:34887
nickel dichloride
5 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
27.0 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
6 Glycine
C2H5NO2 75.067 g/mol
0.05 MILLIMOLAR CHEBI:15428
glycine
 Cross-references: KEGG:gly
7 Sodium bicarbonate
CHO3.Na 84.006 g/mol
6.0 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
8 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
10.0 MILLIMOLAR CHEBI:3312
calcium dichloride
9 Sodium molybdate
MoO4.2Na 205.916 g/mol
3e-07 MILLIMOLAR CHEBI:75215
sodium molybdate (anhydrous)
10 Iron(III) chloride 0.000117 MILLIMOLAR
11 Zinc sulfate
O4S.Zn 161.453 g/mol
8e-07 MILLIMOLAR CHEBI:35176
zinc sulfate
12 Manganese chloride
Cl2Mn 125.844 g/mol
9e-06 MILLIMOLAR CHEBI:63041
manganese(II) chloride
13 2-Aminoethylphosphonate 0.001 MILLIMOLAR Cross-references: KEGG:2ameph
14 Potassium chloride
Cl.K 74.551 g/mol
9.0 MILLIMOLAR CHEBI:32588
potassium chloride
15 DL-methionine
C5H11NO2S 149.215 g/mol
0.05 MILLIMOLAR CHEBI:16811
methionine
16 Cobalt chloride
2Cl.Co 129.839 g/mol
5e-07 MILLIMOLAR CHEBI:35696
cobalt dichloride
17 Sodium chloride
Cl.Na 58.443 g/mol
481.0 MILLIMOLAR CHEBI:26710
sodium chloride
18 Ammonium sulfate
2H4N.O4S 132.141 g/mol
0.4 MILLIMOLAR CHEBI:62946
ammonium sulfate
19 Magnesium sulfate
Mg.O4S 120.368 g/mol
28.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
20 Sodium selenite
2Na.O3Se 172.937 g/mol
1e-06 MILLIMOLAR CHEBI:48843
disodium selenite

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:47.105865Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 483, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:38.164337Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:48.638946Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007391
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:06.986221+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'AMS1 (Pyruvate+Glycine" to "ams1_pyruvate_glycine" for consistent matching
2026-06-05T07:40:29.525228+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T04:05:56.167674+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:483's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM.
2026-08-31T06:17:08.421657+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.